Endothelial Cell Phenotypes Demonstrate Different Metabolic Patterns and Predict Mortality in Trauma Patients

Hanne H Henriksen, Igor Marín de Mas, Lars K Nielsen, Joseph Krocker, Jakob Stensballe, Sigurður T Karvelsson, Niels H Secher, Óttar Rolfsson, Charles E Wade, Pär I Johansson*

*Corresponding author af dette arbejde

Abstract

In trauma patients, shock-induced endotheliopathy (SHINE) is associated with a poor prognosis. We have previously identified four metabolic phenotypes in a small cohort of trauma patients (N = 20) and displayed the intracellular metabolic profile of the endothelial cell by integrating quantified plasma metabolomic profiles into a genome-scale metabolic model (iEC-GEM). A retrospective observational study of 99 trauma patients admitted to a Level 1 Trauma Center. Mass spectrometry was conducted on admission samples of plasma metabolites. Quantified metabolites were analyzed by computational network analysis of the iEC-GEM. Four plasma metabolic phenotypes (A-D) were identified, of which phenotype D was associated with an increased injury severity score (p < 0.001); 90% (91.6%) of the patients who died within 72 h possessed this phenotype. The inferred EC metabolic patterns were found to be different between phenotype A and D. Phenotype D was unable to maintain adequate redox homeostasis. We confirm that trauma patients presented four metabolic phenotypes at admission. Phenotype D was associated with increased mortality. Different EC metabolic patterns were identified between phenotypes A and D, and the inability to maintain adequate redox balance may be linked to the high mortality.

OriginalsprogEngelsk
Artikelnummer2257
TidsskriftInternational Journal of Molecular Sciences
Vol/bind24
Udgave nummer3
ISSN1661-6596
DOI
StatusUdgivet - 23 jan. 2023

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