Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome

Kajsa Kajander; Eveliina Myllyluoma; Sinikka Kyrönpalo; Martin Rasmussen; Pentti Sipponen; Ismo Mattila; Tuulikki Seppänen-Laakso; Heikki Vapaatalo; Matej Oresic; Riitta Korpela

Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome

Kajsa Kajander, Eveliina Myllyluoma, Sinikka Kyrönpalo, Martin Rasmussen, Pentti Sipponen, Ismo Mattila, Tuulikki Seppänen-Laakso, Heikki Vapaatalo, Matej Oresic, Riitta Korpela

37 Citationer (Scopus)

Abstrakt

AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.

METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC x GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.

RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).

CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

Originalsprog	Engelsk
Tidsskrift	World Journal of Gastroenterology
Vol/bind	15
Udgave nummer	48
Sider (fra-til)	6068-74
Antal sider	7
ISSN	1007-9327
Status	Udgivet - 28 dec. 2009
Udgivet eksternt	Ja

Citationsformater

@article{db3bec6f64584bc4ac0dfe07f3b02b46,

title = "Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome",

abstract = "AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC x GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.",

keywords = "Adult, Biopsy, Case-Control Studies, Female, Humans, Intestinal Mucosa/metabolism, Irritable Bowel Syndrome/metabolism, Lipid Metabolism, Male, Metabolomics, Middle Aged",

author = "Kajsa Kajander and Eveliina Myllyluoma and Sinikka Kyr{\"o}npalo and Martin Rasmussen and Pentti Sipponen and Ismo Mattila and Tuulikki Sepp{\"a}nen-Laakso and Heikki Vapaatalo and Matej Oresic and Riitta Korpela",

year = "2009",

month = dec,

day = "28",

language = "English",

volume = "15",

pages = "6068--74",

journal = "World Journal of Gastroenterology",

issn = "1007-9327",

publisher = "W J G Press",

number = "48",

}

TY - JOUR

T1 - Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome

AU - Kajander, Kajsa

AU - Myllyluoma, Eveliina

AU - Kyrönpalo, Sinikka

AU - Rasmussen, Martin

AU - Sipponen, Pentti

AU - Mattila, Ismo

AU - Seppänen-Laakso, Tuulikki

AU - Vapaatalo, Heikki

AU - Oresic, Matej

AU - Korpela, Riitta

PY - 2009/12/28

Y1 - 2009/12/28

N2 - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC x GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

AB - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC x GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

KW - Adult

KW - Biopsy

KW - Case-Control Studies

KW - Female

KW - Humans

KW - Intestinal Mucosa/metabolism

KW - Irritable Bowel Syndrome/metabolism

KW - Lipid Metabolism

KW - Male

KW - Metabolomics

KW - Middle Aged

M3 - Journal article

C2 - 20027679

VL - 15

SP - 6068

EP - 6074

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 48

ER -

Elevated pro-inflammatory and lipotoxic mucosal lipids characterise irritable bowel syndrome

Abstrakt

Fingeraftryk

Citationsformater