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Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes

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Dela, F, Ingersen, A, Andersen, NB, Nielsen, MB, Petersen, HHH, Hansen, CN, Larsen, S, Wojtaszewski, J & Helge, JW 2019, 'Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes', Acta Physiologica, bind 226, nr. 2, s. e13245. https://doi.org/10.1111/apha.13245

APA

Dela, F., Ingersen, A., Andersen, N. B., Nielsen, M. B., Petersen, H. H. H., Hansen, C. N., Larsen, S., Wojtaszewski, J., & Helge, J. W. (2019). Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes. Acta Physiologica, 226(2), e13245. https://doi.org/10.1111/apha.13245

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Author

Dela, Flemming ; Ingersen, Arthur ; Andersen, Nynne B ; Nielsen, Maria B ; Petersen, Helga H H ; Hansen, Christina N ; Larsen, Steen ; Wojtaszewski, Jørgen ; Helge, Jørn Wulff. / Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes. I: Acta Physiologica. 2019 ; Bind 226, Nr. 2. s. e13245.

Bibtex

@article{51a9603b1f2849a0900cfba1621cf04c,
title = "Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes",
abstract = "AIM: To examine the effect of high-intensity interval training (HIIT) on glucose clearance rates in skeletal muscle and explore the mechanism within the muscle.METHODS: Ten males with type 2 diabetes mellitus (T2DM) and ten matched healthy subjects performed 2 weeks of one-legged HIIT (total of eight sessions, each comprised of 10 × 1 minute ergometer bicycle exercise at >80% of maximal heart rate, interspersed with one min of rest). Insulin sensitivity was assessed by an isoglycaemic, hyperinsulinaemic clamp combined with arteriovenous leg balance technique of the trained (T) and the untrained (UT) leg and muscle biopsies of both legs.RESULTS: Insulin-stimulated glucose clearance in T legs was ~30% higher compared with UT legs in both groups due to increased blood flow in T vs UT legs and maintained glucose extraction. With each training session, muscle glycogen content decreased only in the training leg, and after the training, glycogen synthase and citrate synthase activities were higher in T vs UT legs. No major changes occurred in the expression of proteins in the insulin signalling cascade. Mitochondrial respiratory capacity was similar in T2DM and healthy subjects, and unchanged by HIIT.CONCLUSION: HIIT improves skeletal muscle insulin sensitivity. With HIIT, the skeletal muscle of patients with T2DM becomes just as insulin sensitive as untrained muscle in healthy subjects. The mechanism includes oscillations in muscle glycogen stores and a maintained ability to extract glucose from the blood in the face of increased blood flow in the trained leg.",
author = "Flemming Dela and Arthur Ingersen and Andersen, {Nynne B} and Nielsen, {Maria B} and Petersen, {Helga H H} and Hansen, {Christina N} and Steen Larsen and J{\o}rgen Wojtaszewski and Helge, {J{\o}rn Wulff}",
note = "{\textcopyright} 2018 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.",
year = "2019",
month = jun,
doi = "10.1111/apha.13245",
language = "English",
volume = "226",
pages = "e13245",
journal = "Acta Physiologica",
issn = "1748-1708",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Effects of one-legged high-intensity interval training on insulin-mediated skeletal muscle glucose homeostasis in patients with type 2 diabetes

AU - Dela, Flemming

AU - Ingersen, Arthur

AU - Andersen, Nynne B

AU - Nielsen, Maria B

AU - Petersen, Helga H H

AU - Hansen, Christina N

AU - Larsen, Steen

AU - Wojtaszewski, Jørgen

AU - Helge, Jørn Wulff

N1 - © 2018 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

PY - 2019/6

Y1 - 2019/6

N2 - AIM: To examine the effect of high-intensity interval training (HIIT) on glucose clearance rates in skeletal muscle and explore the mechanism within the muscle.METHODS: Ten males with type 2 diabetes mellitus (T2DM) and ten matched healthy subjects performed 2 weeks of one-legged HIIT (total of eight sessions, each comprised of 10 × 1 minute ergometer bicycle exercise at >80% of maximal heart rate, interspersed with one min of rest). Insulin sensitivity was assessed by an isoglycaemic, hyperinsulinaemic clamp combined with arteriovenous leg balance technique of the trained (T) and the untrained (UT) leg and muscle biopsies of both legs.RESULTS: Insulin-stimulated glucose clearance in T legs was ~30% higher compared with UT legs in both groups due to increased blood flow in T vs UT legs and maintained glucose extraction. With each training session, muscle glycogen content decreased only in the training leg, and after the training, glycogen synthase and citrate synthase activities were higher in T vs UT legs. No major changes occurred in the expression of proteins in the insulin signalling cascade. Mitochondrial respiratory capacity was similar in T2DM and healthy subjects, and unchanged by HIIT.CONCLUSION: HIIT improves skeletal muscle insulin sensitivity. With HIIT, the skeletal muscle of patients with T2DM becomes just as insulin sensitive as untrained muscle in healthy subjects. The mechanism includes oscillations in muscle glycogen stores and a maintained ability to extract glucose from the blood in the face of increased blood flow in the trained leg.

AB - AIM: To examine the effect of high-intensity interval training (HIIT) on glucose clearance rates in skeletal muscle and explore the mechanism within the muscle.METHODS: Ten males with type 2 diabetes mellitus (T2DM) and ten matched healthy subjects performed 2 weeks of one-legged HIIT (total of eight sessions, each comprised of 10 × 1 minute ergometer bicycle exercise at >80% of maximal heart rate, interspersed with one min of rest). Insulin sensitivity was assessed by an isoglycaemic, hyperinsulinaemic clamp combined with arteriovenous leg balance technique of the trained (T) and the untrained (UT) leg and muscle biopsies of both legs.RESULTS: Insulin-stimulated glucose clearance in T legs was ~30% higher compared with UT legs in both groups due to increased blood flow in T vs UT legs and maintained glucose extraction. With each training session, muscle glycogen content decreased only in the training leg, and after the training, glycogen synthase and citrate synthase activities were higher in T vs UT legs. No major changes occurred in the expression of proteins in the insulin signalling cascade. Mitochondrial respiratory capacity was similar in T2DM and healthy subjects, and unchanged by HIIT.CONCLUSION: HIIT improves skeletal muscle insulin sensitivity. With HIIT, the skeletal muscle of patients with T2DM becomes just as insulin sensitive as untrained muscle in healthy subjects. The mechanism includes oscillations in muscle glycogen stores and a maintained ability to extract glucose from the blood in the face of increased blood flow in the trained leg.

U2 - 10.1111/apha.13245

DO - 10.1111/apha.13245

M3 - Journal article

C2 - 30585698

VL - 226

SP - e13245

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 2

ER -

ID: 59397148