Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Effects of an 8-weeks erythropoietin treatment on mitochondrial and whole body fat oxidation capacity during exercise in healthy males

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Performance changes during the off-season period in football players - Effects of age and previous hamstring injury

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Differential time responses in inflammatory and oxidative stress markers after a marathon: An observational study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The impact of start strategy on start performance in alpine skiing exists on flat, but not on steep inclines

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Effects of the Nordic Hamstring exercise on sprint capacity in male football players: a randomized controlled trial

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Advanced Electrocardiogram Analysis in the Amitriptyline-poisoned Pig Treated with Activated Charcoal Haemoperfusion

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Effects of low-dose recombinant human erythropoietin treatment on cognitive performance

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Citrate, a Ubiquitous Key Metabolite with Regulatory Function in the CNS

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Abstract The present investigation was performed to elucidate if the non-erythropoietic ergogenic effect of a recombinant erythropoietin treatment results in an impact on skeletal muscle mitochondrial and whole body fatty acid oxidation capacity during exercise, myoglobin concentration and angiogenesis. Recombinant erythropoietin was administered by subcutaneous injections (5000 IU) in six healthy male volunteers (aged 21 ± 2 years; fat mass 18.5 ± 2.3%) over 8 weeks. The participants performed two graded cycle ergometer exercise tests before and after the intervention where VO2max and maximal fat oxidation were measured. Biopsies of the vastus lateralis muscle were obtained before and after the intervention. Recombinant erythropoietin treatment increased mitochondrial O2 flux during ADP stimulated state 3 respiration in the presence of complex I and II substrates (malate, glutamate, pyruvate, succinate) with additional electron input from β-oxidation (octanoylcarnitine) (from 60 ± 13 to 87 ± 24 pmol · s(-1) · mg(-1) P < 0.01). β-hydroxy-acyl-CoA-dehydrogenase activity was higher after treatment (P < 0.05), whereas citrate synthase activity also tended to increase (P = 0.06). Total myoglobin increased by 16.5% (P < 0.05). Capillaries per muscle area tended to increase (P = 0.07), whereas capillaries per fibre as well as the total expression of vascular endothelial growth factor remained unchanged. Whole body maximal fat oxidation was not increased after treatment. Eight weeks of recombinant erythropoietin treatment increases mitochondrial fatty acid oxidation capacity and myoglobin concentration without any effect on whole body maximal fat oxidation.

OriginalsprogEngelsk
TidsskriftJournal of Sports Sciences
Vol/bind33
Udgave nummer6
Sider (fra-til)570-8
Antal sider9
ISSN0264-0414
DOI
StatusUdgivet - 2015

ID: 45273893