TY - JOUR
T1 - Effect of whey protein supplementation on sperm quality and fertility in male mice
AU - Ketheeswaran, Shathmigha
AU - Pors, Susanne Elisabeth
AU - Zuniga Jara, Lenin Arturo
AU - Lemser, Camilla Engel
AU - Høj, Terese Kimmie
AU - Bøtkjær, Jane Alrø
AU - Christensen, Preben
AU - Humaidan, Peter
AU - Kristensen, Stine Gry
N1 - Copyright © 2020 Elsevier Ltd. All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - Protein supplements are a billion-dollar industry and the intake of these supplements is increasing, especially among young men. However, little is known about whether consumption of these products affects the reproductive health. The aim of this study was to assess the effect of whey protein supplementation on the sperm quality and reproductive health of male mice. A total of 48 male NMRI mice were fed with either plain tap water or a high dose of whey protein (Whey100, BodyLab) supplemented in the drinking water for 3 months. Mice was individually housed with two female mice for five days and reproductive parameters were assessed. DNA fragmentation index (DFI) was assessed at 0 h and 4 h of in vitro incubation using a sperm DNA integrity test (SDI®-test). No significant differences were detected between the groups in the epididymal sperm count, sperm motility, DFI, oxidation-reduction potential (ORP), serum testosterone, body and seminal vesicles weights, relative testis and epididymal weights, testicular morphology, number of impregnated females, or litter size. No correlation was found between ORP and DFI. These results suggest that the highest recommended human dose of whey protein supplementation do not significantly impair the sperm quality and fertility in male mice.
AB - Protein supplements are a billion-dollar industry and the intake of these supplements is increasing, especially among young men. However, little is known about whether consumption of these products affects the reproductive health. The aim of this study was to assess the effect of whey protein supplementation on the sperm quality and reproductive health of male mice. A total of 48 male NMRI mice were fed with either plain tap water or a high dose of whey protein (Whey100, BodyLab) supplemented in the drinking water for 3 months. Mice was individually housed with two female mice for five days and reproductive parameters were assessed. DNA fragmentation index (DFI) was assessed at 0 h and 4 h of in vitro incubation using a sperm DNA integrity test (SDI®-test). No significant differences were detected between the groups in the epididymal sperm count, sperm motility, DFI, oxidation-reduction potential (ORP), serum testosterone, body and seminal vesicles weights, relative testis and epididymal weights, testicular morphology, number of impregnated females, or litter size. No correlation was found between ORP and DFI. These results suggest that the highest recommended human dose of whey protein supplementation do not significantly impair the sperm quality and fertility in male mice.
KW - DNA fragmentation Index
KW - Fertility
KW - Male mice
KW - Oxidative stress
KW - Protein supplementation
KW - Sperm quality
UR - http://www.scopus.com/inward/record.url?scp=85083585520&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2020.111366
DO - 10.1016/j.fct.2020.111366
M3 - Journal article
C2 - 32315685
SN - 0278-6915
VL - 141
SP - 111366
JO - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
M1 - 111366
ER -