TY - JOUR
T1 - Effect of thyroid hormones on vasoactive intestinal polypeptide gene expression in the rat cerebral cortex and anterior pituitary
AU - Buhl, T
AU - Georg, B
AU - Nilsson, C
AU - Mikkelsen, J D
AU - Wulff, B S
AU - Fahrenkrug, J
PY - 1995/2/14
Y1 - 1995/2/14
N2 - We report here data on the expression of the various sequences derived from the prepro-vasoactive intestinal polypeptide (VIP) precursor and VIP mRNA in the anterior pituitary gland and cerebral cortex of hypothyroid and hyperthyroid rats. Using specific antisera to each of the prepro-VIP sequences we demonstrated an increase of all prepro-VIP derived sequences, and accordingly, found that the number of cells expressing each of these sequences were markedly augmented in the anterior pituitary of the hypothyroid rats. This was accompanied by a marked increase in VIP mRNA. In the cerebral cortex of the hypothyroid rats no changes were observed. In the pituitary of hyperthyroid animals a significant decrease was seen for prepro-VIP 22-79, VIP and prepro-VIP 156-170, whereas in the cerebral cortex a significant increase was observed for prepro-VIP 22-79, PHI and VIP. We were not able to demonstrate any changes in VIP mRNA in the cerebral cortex or pituitary of the hyperthyroid rats. Gel permeation chromatography and reverse-phase HPLC of extracts from cerebral cortex showed elution profiles identical to the synthetic counterparts. The reported data provide further evidence of a tissue-specific expression and regulation of the VIP gene products.
AB - We report here data on the expression of the various sequences derived from the prepro-vasoactive intestinal polypeptide (VIP) precursor and VIP mRNA in the anterior pituitary gland and cerebral cortex of hypothyroid and hyperthyroid rats. Using specific antisera to each of the prepro-VIP sequences we demonstrated an increase of all prepro-VIP derived sequences, and accordingly, found that the number of cells expressing each of these sequences were markedly augmented in the anterior pituitary of the hypothyroid rats. This was accompanied by a marked increase in VIP mRNA. In the cerebral cortex of the hypothyroid rats no changes were observed. In the pituitary of hyperthyroid animals a significant decrease was seen for prepro-VIP 22-79, VIP and prepro-VIP 156-170, whereas in the cerebral cortex a significant increase was observed for prepro-VIP 22-79, PHI and VIP. We were not able to demonstrate any changes in VIP mRNA in the cerebral cortex or pituitary of the hyperthyroid rats. Gel permeation chromatography and reverse-phase HPLC of extracts from cerebral cortex showed elution profiles identical to the synthetic counterparts. The reported data provide further evidence of a tissue-specific expression and regulation of the VIP gene products.
KW - Animals
KW - Blotting, Northern
KW - Cerebral Cortex/metabolism
KW - Chromatography, Gel
KW - Chromatography, High Pressure Liquid
KW - Gene Expression/drug effects
KW - Hyperthyroidism/metabolism
KW - Hypothyroidism/metabolism
KW - Immunohistochemistry
KW - In Situ Hybridization
KW - Male
KW - Pituitary Gland, Anterior/metabolism
KW - Protein Precursors/metabolism
KW - RNA, Messenger/metabolism
KW - Radioimmunoassay
KW - Rats
KW - Rats, Sprague-Dawley
KW - Thyroid Hormones/pharmacology
KW - Vasoactive Intestinal Peptide/genetics
U2 - 10.1016/0167-0115(94)00112-b
DO - 10.1016/0167-0115(94)00112-b
M3 - Journal article
C2 - 7761623
SN - 0167-0115
VL - 55
SP - 237
EP - 251
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 3
ER -