Effect of fecal microbiota transplantation route of administration on gut colonization and host response in preterm pigs

Anders Brunse, Lena Martin, Torben Sølbeck Rasmussen, Lars Christensen, Malene Skovsted Cilieborg, Maria Wiese, Bekzod Khakimov, Robert Pieper, Dennis Sandris Nielsen, Per Torp Sangild, Thomas Thymann

Abstract

This study examined gut colonization patterns and host responses to fecal microbiota transplantation (FMT) by different administration routes after preterm birth. In two separate experiments, cesarean-delivered, preterm pigs were administered combined oral + rectal, or exclusively rectal donor feces, and compared with saline controls. After 5 days, stomach and colon bacterial compositions were determined by 16S rRNA gene amplicon sequencing, and organic acid metabolites measured. Further, gut pathology, mucosa bacterial adherence, and goblet cell density were assessed. FMT increased the relative abundance of obligate anaerobes in the colon without affecting total bacterial load. Bacteroides colonized recipients despite low abundance in the donor feces, whereas highly abundant Prevotella and Ruminococcaceae did not. Further, FMT changed carbohydrate metabolism from lactate to propionate production thereby increasing colonic pH. Besides, FMT preserved goblet cell mucin stores and reduced necrotizing enterocolitis incidence. Only rectal FMT increased the stomach-to-colon pH gradient and resistance to mucosa bacterial adhesion. Conversely, oral + rectal FMT increased bacterial adhesion, internal organ colonization, and overall mortality. Our results uncovered distinctions in bacterial colonization patterns along the gastrointestinal tract, as well as host tolerability between oral and rectal FMT administration in preterm newborns. Besides, FMT showed the potential to prevent necrotizing enterocolitis.

OriginalsprogEngelsk
TidsskriftISME Journal
Vol/bind13
Udgave nummer3
Sider (fra-til)720-733
Antal sider14
ISSN1751-7362
DOI
StatusUdgivet - mar. 2019

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