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Effect of anti-carbohydrate antibodies on HIV infection in a monocytic cell line (U937).

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@article{cf191559c6184302b37049063f621d5e,
title = "Effect of anti-carbohydrate antibodies on HIV infection in a monocytic cell line (U937).",
abstract = "Monoclonal antibodies (mAbs) against carbohydrate epitopes of gp120 have recently been found to inhibit HIV infection of lymphocytes in vitro thereby opening new possibilities for vaccine considerations. Antibody-dependent enhancement of infection has however come increasingly into focus. This study therefore investigated the neutralization of HIV in a monocytic cell line (U937) using mAbs against these carbohydrate gp120-epitopes. While antibodies against one of the epitopes (AI) neutralized infection of U937 cells despite binding to the Fc-receptor, one mAb against the sialosyl-Tn epitope enhanced infection. This enhancement was independent of complement and could be blocked by mAb Leu3a against the CD4-receptor. The study indicated that enhancement of infection in monocytic cells can occur by the same anti-carbohydrate antibodies that neutralize infection in lymphocytes, and that antibody mediated enhancement may depend on location of the epitope on gp120 rather than whether the antibody binds Fc-receptors.",
author = "Hansen, {J E} and C Nielsen and H Clausen and Mathiesen, {Lars Reinhardt} and Nielsen, {Jens Ole}",
year = "1991",
language = "English",
volume = "16",
pages = "233--242",
journal = "Antiviral Research",
issn = "0166-3542",
publisher = "Elsevier BV",
number = "3",

}

RIS

TY - JOUR

T1 - Effect of anti-carbohydrate antibodies on HIV infection in a monocytic cell line (U937).

AU - Hansen, J E

AU - Nielsen, C

AU - Clausen, H

AU - Mathiesen, Lars Reinhardt

AU - Nielsen, Jens Ole

PY - 1991

Y1 - 1991

N2 - Monoclonal antibodies (mAbs) against carbohydrate epitopes of gp120 have recently been found to inhibit HIV infection of lymphocytes in vitro thereby opening new possibilities for vaccine considerations. Antibody-dependent enhancement of infection has however come increasingly into focus. This study therefore investigated the neutralization of HIV in a monocytic cell line (U937) using mAbs against these carbohydrate gp120-epitopes. While antibodies against one of the epitopes (AI) neutralized infection of U937 cells despite binding to the Fc-receptor, one mAb against the sialosyl-Tn epitope enhanced infection. This enhancement was independent of complement and could be blocked by mAb Leu3a against the CD4-receptor. The study indicated that enhancement of infection in monocytic cells can occur by the same anti-carbohydrate antibodies that neutralize infection in lymphocytes, and that antibody mediated enhancement may depend on location of the epitope on gp120 rather than whether the antibody binds Fc-receptors.

AB - Monoclonal antibodies (mAbs) against carbohydrate epitopes of gp120 have recently been found to inhibit HIV infection of lymphocytes in vitro thereby opening new possibilities for vaccine considerations. Antibody-dependent enhancement of infection has however come increasingly into focus. This study therefore investigated the neutralization of HIV in a monocytic cell line (U937) using mAbs against these carbohydrate gp120-epitopes. While antibodies against one of the epitopes (AI) neutralized infection of U937 cells despite binding to the Fc-receptor, one mAb against the sialosyl-Tn epitope enhanced infection. This enhancement was independent of complement and could be blocked by mAb Leu3a against the CD4-receptor. The study indicated that enhancement of infection in monocytic cells can occur by the same anti-carbohydrate antibodies that neutralize infection in lymphocytes, and that antibody mediated enhancement may depend on location of the epitope on gp120 rather than whether the antibody binds Fc-receptors.

M3 - Journal article

VL - 16

SP - 233

EP - 242

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

IS - 3

ER -

ID: 32528355