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During infancy low levels of follicle-stimulating hormone may result in high rate of germ cell apoptosis

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@article{1844155d8dbc47c5a905e1b2b131d5a9,
title = "During infancy low levels of follicle-stimulating hormone may result in high rate of germ cell apoptosis",
abstract = "PURPOSE: It has been suggested that follicle-stimulating hormone (FSH) plays a role in preventing germ cell apoptosis. We aimed to compare apoptotic rate of boys with cryptorchidism having different levels of FSH in order to investigate its role in apoptosis.METHODS: Hormonal profiles and testicular biopsies from 30 boys with unilateral cryptorchidism (age range: 4-14 months) were included. Based on FSH level, the boys were grouped into three (3 × 10) having high (>97.5percentile), low (<2.5percentile), or within normal range. Sections underwent immunohistochemical staining to analyze the number of germ cells and type A dark spermatogonia per cross-sectional tubule. One section was co-stained with immunofluorescent antibodies against an apoptotic marker (cleaved caspase-3), proliferation marker (Ki67), Sertoli cell marker (anti-M{\"u}llerian hormone) and processed by confocal imaging for analysis. Germ cell apoptosis was calculated as the apoptosis index (percentage caspase-3+ germ cells/total germ cell number).RESULTS: Fifty percent (5/10) of the boys with low FSH had an apoptosis index above 90% compared with 15% (3/20) of the boys with normal or high FSH (p = 0.04). Caspase-3+ germ cells were most likely to be located on the basement membrane (p<0.05).CONCLUSION: Our findings lead to trends proposing that FSH may play a role in preventing apoptosis.TYPE OF STUDY: Prognosis Study LEVEL OF EVIDENCE: III.",
keywords = "Apoptosis, Cryptorchidism, Follicle-stimulating hormone, Germ cells, Gonocyte transformation",
author = "Simone Hildorf and Dina Cortes and J{\o}rgen Thorup and Erik Clasen-Linde and John Hutson and Ruili Li",
note = "Copyright {\textcopyright} 2021 Elsevier Inc. All rights reserved.",
year = "2021",
month = dec,
doi = "10.1016/j.jpedsurg.2021.02.012",
language = "English",
volume = "56",
pages = "2399--2406",
journal = "Journal of Pediatric Surgery",
issn = "0022-3468",
publisher = "W.B./Saunders Co",
number = "12",

}

RIS

TY - JOUR

T1 - During infancy low levels of follicle-stimulating hormone may result in high rate of germ cell apoptosis

AU - Hildorf, Simone

AU - Cortes, Dina

AU - Thorup, Jørgen

AU - Clasen-Linde, Erik

AU - Hutson, John

AU - Li, Ruili

N1 - Copyright © 2021 Elsevier Inc. All rights reserved.

PY - 2021/12

Y1 - 2021/12

N2 - PURPOSE: It has been suggested that follicle-stimulating hormone (FSH) plays a role in preventing germ cell apoptosis. We aimed to compare apoptotic rate of boys with cryptorchidism having different levels of FSH in order to investigate its role in apoptosis.METHODS: Hormonal profiles and testicular biopsies from 30 boys with unilateral cryptorchidism (age range: 4-14 months) were included. Based on FSH level, the boys were grouped into three (3 × 10) having high (>97.5percentile), low (<2.5percentile), or within normal range. Sections underwent immunohistochemical staining to analyze the number of germ cells and type A dark spermatogonia per cross-sectional tubule. One section was co-stained with immunofluorescent antibodies against an apoptotic marker (cleaved caspase-3), proliferation marker (Ki67), Sertoli cell marker (anti-Müllerian hormone) and processed by confocal imaging for analysis. Germ cell apoptosis was calculated as the apoptosis index (percentage caspase-3+ germ cells/total germ cell number).RESULTS: Fifty percent (5/10) of the boys with low FSH had an apoptosis index above 90% compared with 15% (3/20) of the boys with normal or high FSH (p = 0.04). Caspase-3+ germ cells were most likely to be located on the basement membrane (p<0.05).CONCLUSION: Our findings lead to trends proposing that FSH may play a role in preventing apoptosis.TYPE OF STUDY: Prognosis Study LEVEL OF EVIDENCE: III.

AB - PURPOSE: It has been suggested that follicle-stimulating hormone (FSH) plays a role in preventing germ cell apoptosis. We aimed to compare apoptotic rate of boys with cryptorchidism having different levels of FSH in order to investigate its role in apoptosis.METHODS: Hormonal profiles and testicular biopsies from 30 boys with unilateral cryptorchidism (age range: 4-14 months) were included. Based on FSH level, the boys were grouped into three (3 × 10) having high (>97.5percentile), low (<2.5percentile), or within normal range. Sections underwent immunohistochemical staining to analyze the number of germ cells and type A dark spermatogonia per cross-sectional tubule. One section was co-stained with immunofluorescent antibodies against an apoptotic marker (cleaved caspase-3), proliferation marker (Ki67), Sertoli cell marker (anti-Müllerian hormone) and processed by confocal imaging for analysis. Germ cell apoptosis was calculated as the apoptosis index (percentage caspase-3+ germ cells/total germ cell number).RESULTS: Fifty percent (5/10) of the boys with low FSH had an apoptosis index above 90% compared with 15% (3/20) of the boys with normal or high FSH (p = 0.04). Caspase-3+ germ cells were most likely to be located on the basement membrane (p<0.05).CONCLUSION: Our findings lead to trends proposing that FSH may play a role in preventing apoptosis.TYPE OF STUDY: Prognosis Study LEVEL OF EVIDENCE: III.

KW - Apoptosis

KW - Cryptorchidism

KW - Follicle-stimulating hormone

KW - Germ cells

KW - Gonocyte transformation

UR - http://www.scopus.com/inward/record.url?scp=85102044772&partnerID=8YFLogxK

U2 - 10.1016/j.jpedsurg.2021.02.012

DO - 10.1016/j.jpedsurg.2021.02.012

M3 - Journal article

C2 - 33691939

VL - 56

SP - 2399

EP - 2406

JO - Journal of Pediatric Surgery

JF - Journal of Pediatric Surgery

SN - 0022-3468

IS - 12

ER -

ID: 64228218