Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia

Mikael Knutsson; Lars Edvinsson

Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia

86 Citationer (Scopus)

Abstract

The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.

Originalsprog	Engelsk
Tidsskrift	NeuroReport
Vol/bind	13
Udgave nummer	4
Sider (fra-til)	507-9
Antal sider	3
ISSN	0959-4965
Status	Udgivet - 25 mar. 2002
Udgivet eksternt	Ja

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Citationsformater

@article{1896695973c94ab8855f0e3ca1e2d4b3,

title = "Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia",

abstract = "The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80\%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.",

keywords = "Adult, Cerebral Arteries, Endothelium, Vascular, Humans, RNA, Messenger, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I, Receptors, Pituitary Hormone, Receptors, Vasoactive Intestinal Peptide, Receptors, Vasoactive Intestinal Peptide, Type II, Receptors, Vasoactive Intestinal Polypeptide, Type I, Superior Cervical Ganglion, Trigeminal Ganglion",

author = "Mikael Knutsson and Lars Edvinsson",

year = "2002",

month = mar,

day = "25",

language = "English",

volume = "13",

pages = "507--9",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "4",

}

TY - JOUR

T1 - Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia

AU - Knutsson, Mikael

AU - Edvinsson, Lars

PY - 2002/3/25

Y1 - 2002/3/25

N2 - The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.

AB - The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.

KW - Adult

KW - Cerebral Arteries

KW - Endothelium, Vascular

KW - Humans

KW - RNA, Messenger

KW - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide

KW - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I

KW - Receptors, Pituitary Hormone

KW - Receptors, Vasoactive Intestinal Peptide

KW - Receptors, Vasoactive Intestinal Peptide, Type II

KW - Receptors, Vasoactive Intestinal Polypeptide, Type I

KW - Superior Cervical Ganglion

KW - Trigeminal Ganglion

UR - https://www.scopus.com/pages/publications/0037171104

M3 - Journal article

C2 - 11930171

SN - 0959-4965

VL - 13

SP - 507

EP - 509

JO - NeuroReport

JF - NeuroReport

IS - 4

ER -

Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia

Abstract

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