TY - JOUR
T1 - Distribution of mRNA for VIP and PACAP receptors in human cerebral arteries and cranial ganglia
AU - Knutsson, Mikael
AU - Edvinsson, Lars
PY - 2002/3/25
Y1 - 2002/3/25
N2 - The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.
AB - The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 (PAC1) and vasoactive intestinal polypeptide (VIP) types 1 and 2 (VPAC1 and VPAC2, respectively) receptors was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in human cerebral arteries and in trigeminal, otic, sphenopalatine and superior cervical ganglia. RT-PCR products of the expected sizes were detected in the arteries, in both the presence and absence of endothelium. In the majority (80%) of the trigeminal, otic and superior cervical ganglia, mRNA for all three receptors were found (n = 5). However, in the sphenopalatine ganglion neither VPAC2 nor PAC1 was detected (n = 2). This finding indicates the occurrence of both prejunctional (ganglia) and postjunctional (vessels) location of VIP and PACAP receptors.
KW - Adult
KW - Cerebral Arteries
KW - Endothelium, Vascular
KW - Humans
KW - RNA, Messenger
KW - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
KW - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
KW - Receptors, Pituitary Hormone
KW - Receptors, Vasoactive Intestinal Peptide
KW - Receptors, Vasoactive Intestinal Peptide, Type II
KW - Receptors, Vasoactive Intestinal Polypeptide, Type I
KW - Superior Cervical Ganglion
KW - Trigeminal Ganglion
M3 - Journal article
C2 - 11930171
SN - 0959-4965
VL - 13
SP - 507
EP - 509
JO - NeuroReport
JF - NeuroReport
IS - 4
ER -