Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Disparate changes in kisspeptin and neurokinin B expression in the arcuate nucleus following sex steroid manipulation reveal differential regulation of the two KNDy peptides in rats

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Evidence for glucagon secretion and function within the human gut

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Using a Reporter Mouse to Map Known and Novel Sites of GLP-1 Receptor Expression in Peripheral Tissues of Male Mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Repeated Activation of Noradrenergic Receptors in the Ventromedial Hypothalamus Suppresses the Response to Hypoglycemia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Activin A determines steroid levels and composition in the fetal testis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Kisspeptin, neurokinin B (NKB) and dynorphin A are co-expressed in a population of neurons in the arcuate nucleus (ARC), termed KNDy neurons, which were recently recognized as important elements for the generation of GnRH pulses. However, the topographic distribution of these peptides and their regulated expression by sex steroids are still not well understood. In this study, detailed examination of NKB and kisspeptin immunoreactivity in the rat ARC was carried out, including comparison between sexes, with and without sex steroid replacement. Neurons expressing kisspeptin and NKB were more prominent in the caudal ARC of females, whereas neurons expressing NKB, but not kisspeptin, were the most abundant in the male. Sex steroid manipulation revealed differential regulation of kisspeptin and NKB; while kisspeptin immunoreactive (-ir) cells increased in response to gonadectomy, NKB remained unchanged. Furthermore, the number of NKB-ir cells increased upon sex steroid replacement compared to gonadectomy, while kisspeptin did not, suggesting that sex steroids differently regulate these peptides. In addition, only in females did the density of kisspeptin- and NKB-ir fibers in the ARC increase upon sex steroid replacement in relation to sham and OVX, respectively, suggesting sex-specific regulation of release. In conclusion, our observations reveal sex differences in the number of kisspeptin- and NKB-ir cells, which are more prominent in the caudal ARC. The divergent regulation of kisspeptin and NKB peptide contents in the ARC as function of sex and steroid milieu enlarge our understanding on how these neuropeptides are post-transcriptionally regulated in KNDy neurons.

OriginalsprogEngelsk
TidsskriftEndocrinology
Vol/bind155
Udgave nummer10
Sider (fra-til)3945-55
ISSN0013-7227
DOI
StatusUdgivet - 22 jul. 2014

ID: 44558124