Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Y Wolff; Steffen Eickhardt; Maria Kristin Björnsdottir; Claus Moser; Thomas Bjarnsholt; Niels Høiby; Trine Rolighed Thomsen

doi:10.1128/JCM.00190-13

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Tine Y Wolff, Steffen Eickhardt, Maria Kristin Björnsdottir, Claus Moser, Thomas Bjarnsholt, Niels Høiby, Trine Rolighed Thomsen

Afdeling for Klinisk Mikrobiologi DIA

7 Citationer (Scopus)

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	51
Udgave nummer	4
Sider (fra-til)	1281-4
Antal sider	4
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.00190-13
Status	Udgivet - apr. 2013

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title = "Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections",

abstract = "In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.",

keywords = "Adult, Aged, Bacterial Infections, Coinfection, Computational Biology, Female, Humans, Male, Middle Aged, Molecular Diagnostic Techniques, Sequence Analysis, DNA, Software",

author = "Wolff, \{Tine Y\} and Steffen Eickhardt and Bj{\"o}rnsdottir, \{Maria Kristin\} and Claus Moser and Thomas Bjarnsholt and Niels H{\o}iby and Thomsen, \{Trine Rolighed\}",

year = "2013",

month = apr,

doi = "10.1128/JCM.00190-13",

language = "English",

volume = "51",

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TY - JOUR

T1 - Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

AU - Wolff, Tine Y

AU - Eickhardt, Steffen

AU - Björnsdottir, Maria Kristin

AU - Moser, Claus

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Thomsen, Trine Rolighed

PY - 2013/4

Y1 - 2013/4

N2 - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

AB - In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

KW - Adult

KW - Aged

KW - Bacterial Infections

KW - Coinfection

KW - Computational Biology

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Diagnostic Techniques

KW - Sequence Analysis, DNA

KW - Software

UR - https://www.scopus.com/pages/publications/84875818919

U2 - 10.1128/JCM.00190-13

DO - 10.1128/JCM.00190-13

M3 - Journal article

C2 - 23363825

SN - 0095-1137

VL - 51

SP - 1281

EP - 1284

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

Abstract

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