Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

K Bjorn-Mortensen; J Zallet; T Lillebaek; A B Andersen; Sissel Signe Niemann; E M Rasmussen; T A Kohl

doi:10.1128/JCM.00662-15

Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

K Bjorn-Mortensen, J Zallet, T Lillebaek, A B Andersen, Sissel Signe Niemann, E M Rasmussen, T A Kohl

15 Citationer (Scopus)

Abstract

Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	53
Udgave nummer	8
Sider (fra-til)	2716-9
Antal sider	4
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.00662-15
Status	Udgivet - aug. 2015

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10.1128/JCM.00662-15

Citationsformater

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title = "Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing",

abstract = "Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.",

author = "K Bjorn-Mortensen and J Zallet and T Lillebaek and Andersen, {A B} and Niemann, {Sissel Signe} and Rasmussen, {E M} and Kohl, {T A}",

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T1 - Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

AU - Bjorn-Mortensen, K

AU - Zallet, J

AU - Lillebaek, T

AU - Andersen, A B

AU - Niemann, Sissel Signe

AU - Rasmussen, E M

AU - Kohl, T A

PY - 2015/8

Y1 - 2015/8

N2 - Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

AB - Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

U2 - 10.1128/JCM.00662-15

DO - 10.1128/JCM.00662-15

M3 - Journal article

C2 - 26019203

SN - 0095-1137

VL - 53

SP - 2716

EP - 2719

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 8

ER -

Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing

Abstract

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