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Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Differential detection of Human Papillomavirus genotypes and cervical intraepithelial neoplasia by four commercial assays

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Update from a twelve-year nationwide fungaemia surveillance: increasing intrinsic and acquired resistance causes concern

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  2. PREVALENCE OF HUMAN PAPILLOMAVIRUS IN SELF-TAKEN SAMPLES FROM SCREENING NON-ATTENDERS

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  4. A 5' -nuclease genotyping assay for identification of macrolide-resistant Mycoplasma genitalium in clinical specimens

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  1. Prevalence, type distribution, and risk factors for oral HPV in Danish renal transplant recipients

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  2. Clinical Utility of Human Papillomavirus Genotyping in Cervical Cancer Screening: A Systematic Review

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  3. Clinical performance of the HPV-Risk assay on cervical samples in SurePath medium using the VALGENT-4 panel

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. BAYESIAN ANALYSIS OF BASELINE RISK OF CIN2 AND ≥CIN3 BY HPV GENOTYPE IN A EUROPEAN REFERRAL COHORT

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Laboratories can nowadays choose from >100 Human Papillomavirus (HPV) assays for cervical screening. Our previous analysis based on the data from the Danish Horizon study, however, showed that four widely used assays, Hybrid Capture 2 (HC2), cobas, CLART and APTIMA, frequently do not detect the same HPV infections. Here, we determined the characteristics of the concordant (all four assays returning a positive HPV test result) and discordant samples (all other HPV-positive samples) in primary cervical screening at 30-65 years (n=2859) and in a concurrent referral population from the same catchment area (n=885). HPV testing followed the manufacturers' protocols. Women with abnormal cytology were managed according to the routine recommendations. Cytology-normal/HPV-positive women were invited for repeated testing in 18 months. Screening history and histologically confirmed cervical intraepithelial neoplasia (CIN) in 2.5 years after the baseline testing were determined from the national pathology register. HPV-positive women undergoing primary screening having concordant samples were more likely to harbor high-risk infections and less likely to harbor only low-risk infections than women with discordant samples. Additionally, assay signal strengths were substantially higher in concordant samples. More than 80% of ≥CIN2 were found in women with concordant samples, and none where the infection was detected by only one assay. These patterns were similar in the referral population, despite the younger age and more HPV infections. HPV test result discordance identified a cluster of low-risk HPV infections that were hardly ever associated with high-grade CIN and, almost exclusively, represented false-positive screening findings.

OriginalsprogEngelsk
TidsskriftJournal of Clinical Microbiology
Vol/bind54
Udgave nummer11
Sider (fra-til)2669-2675
ISSN0095-1137
DOI
StatusUdgivet - nov. 2016

ID: 48369872