Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Circulating levels of platelet α-granule cytokines in trauma patients

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Inflammatory single nucleotide polymorphisms and the risk of atrial fibrillation: a case control study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The influence of statin treatment on the inflammatory biomarkers YKL-40 and HsCRP in patients with stable coronary artery disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Single nucleotide polymorphisms in inflammatory genes and the risk of early onset of lone atrial fibrillation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Biomarkers of subclinical atherosclerosis in patients with psoriasis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Predictors of Response to Biologics in Patients with Moderate-to-severe Psoriasis: A Danish Nationwide Cohort Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. The role of interleukin-1β in the immune response to contact allergens

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

Vis graf over relationer

OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.

MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).

METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.

RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.

CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.

OriginalsprogEngelsk
TidsskriftInflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN1023-3830
DOI
StatusUdgivet - 31 dec. 2015

ID: 45974954