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Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis

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Harvard

Blauenfeldt, T, Villar-Hernandez, R, García-García, E, Latorre, I, Holm, LL, Muriel-Moreno, B, De Souza-Galvão, ML, Millet, JP, Sabriá, F, Sánchez-Montalva, A, Ruiz-Manzano, J, Pilarte, J, Jiménez, MA, Centeno, C, Torres, C, Molina-Pinargote, I, González-Díaz, YD, Santiago, J, Cantos, A, Prat, C, Andersen, P, Dominguez, J & Ruhwald, M 2020, 'Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis' Journal of Clinical Microbiology, bind 58, nr. 10. https://doi.org/10.1128/JCM.00848-20

APA

CBE

Blauenfeldt T, Villar-Hernandez R, García-García E, Latorre I, Holm LL, Muriel-Moreno B, De Souza-Galvão ML, Millet JP, Sabriá F, Sánchez-Montalva A, Ruiz-Manzano J, Pilarte J, Jiménez MA, Centeno C, Torres C, Molina-Pinargote I, González-Díaz YD, Santiago J, Cantos A, Prat C, Andersen P, Dominguez J, Ruhwald M. 2020. Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis. Journal of Clinical Microbiology. 58(10). https://doi.org/10.1128/JCM.00848-20

MLA

Vancouver

Author

Blauenfeldt, Thomas ; Villar-Hernandez, Raquel ; García-García, Esther ; Latorre, Irene ; Holm, Line Lindebo ; Muriel-Moreno, Beatriz ; De Souza-Galvão, Maria Luiza ; Millet, Joan Pau ; Sabriá, Fina ; Sánchez-Montalva, Adrián ; Ruiz-Manzano, Juan ; Pilarte, Jose ; Jiménez, María A ; Centeno, Carmen ; Torres, Carmen ; Molina-Pinargote, Israel ; González-Díaz, Yoel D ; Santiago, Javier ; Cantos, Adela ; Prat, Cristina ; Andersen, Peter ; Dominguez, Jose ; Ruhwald, Morten. / Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis. I: Journal of Clinical Microbiology. 2020 ; Bind 58, Nr. 10.

Bibtex

@article{c6e2a79135c84d4b926387c3c5dccf01,
title = "Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis",
abstract = "Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 100-fold-higher expression level than IFN-γ, allowing for different analysis platforms, including molecular detection. The PCR technique is already an integrated tool in most TB laboratories and, thus, an obvious platform to turn to. In this case-control study, we investigated the diagnostic sensitivity and specificity of a molecular assay detecting IP-10 mRNA expression following antigen stimulation of a blood sample. We included 89 TB patients and 99 healthy controls. Blood was drawn in QuantiFeron-TB gold in-tube (QFT) assay tubes. Eight hours poststimulation, IP-10 mRNA expression was analyzed, and 20 h poststimulation, IP-10 and IFN-γ protein plasma levels were analyzed using an in-house IP-10 enzyme-linked immunosorbent assay (ELISA) and the official QFT ELISA, respectively. The IP-10 mRNA assay provided high specificity (98{\%}), sensitivity (80{\%}), and area under the concentration-time curve (AUC) (0.97); however, the QFT assay provided a higher overall diagnostic potential, with specificity of 100{\%}, sensitivity of 90{\%}, and AUC of 0.99. The IP-10 protein assay performed on par with the QFT assay, with specificity of 98{\%}, sensitivity of 87{\%}, and AUC of 0.98. We have provided proof of high technical performance of a molecular assay detecting IP-10 mRNA expression. As a diagnostic tool, this assay would gain from further optimization, especially on the kinetics of IP-10 mRNA expression.",
keywords = "IP-10, diagnosis, mRNA, qPCR, tuberculosis",
author = "Thomas Blauenfeldt and Raquel Villar-Hernandez and Esther Garc{\'i}a-Garc{\'i}a and Irene Latorre and Holm, {Line Lindebo} and Beatriz Muriel-Moreno and {De Souza-Galv{\~a}o}, {Maria Luiza} and Millet, {Joan Pau} and Fina Sabri{\'a} and Adri{\'a}n S{\'a}nchez-Montalva and Juan Ruiz-Manzano and Jose Pilarte and Jim{\'e}nez, {Mar{\'i}a A} and Carmen Centeno and Carmen Torres and Israel Molina-Pinargote and Gonz{\'a}lez-D{\'i}az, {Yoel D} and Javier Santiago and Adela Cantos and Cristina Prat and Peter Andersen and Jose Dominguez and Morten Ruhwald",
note = "Copyright {\circledC} 2020 American Society for Microbiology.",
year = "2020",
month = "9",
day = "22",
doi = "10.1128/JCM.00848-20",
language = "English",
volume = "58",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis

AU - Blauenfeldt, Thomas

AU - Villar-Hernandez, Raquel

AU - García-García, Esther

AU - Latorre, Irene

AU - Holm, Line Lindebo

AU - Muriel-Moreno, Beatriz

AU - De Souza-Galvão, Maria Luiza

AU - Millet, Joan Pau

AU - Sabriá, Fina

AU - Sánchez-Montalva, Adrián

AU - Ruiz-Manzano, Juan

AU - Pilarte, Jose

AU - Jiménez, María A

AU - Centeno, Carmen

AU - Torres, Carmen

AU - Molina-Pinargote, Israel

AU - González-Díaz, Yoel D

AU - Santiago, Javier

AU - Cantos, Adela

AU - Prat, Cristina

AU - Andersen, Peter

AU - Dominguez, Jose

AU - Ruhwald, Morten

N1 - Copyright © 2020 American Society for Microbiology.

PY - 2020/9/22

Y1 - 2020/9/22

N2 - Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 100-fold-higher expression level than IFN-γ, allowing for different analysis platforms, including molecular detection. The PCR technique is already an integrated tool in most TB laboratories and, thus, an obvious platform to turn to. In this case-control study, we investigated the diagnostic sensitivity and specificity of a molecular assay detecting IP-10 mRNA expression following antigen stimulation of a blood sample. We included 89 TB patients and 99 healthy controls. Blood was drawn in QuantiFeron-TB gold in-tube (QFT) assay tubes. Eight hours poststimulation, IP-10 mRNA expression was analyzed, and 20 h poststimulation, IP-10 and IFN-γ protein plasma levels were analyzed using an in-house IP-10 enzyme-linked immunosorbent assay (ELISA) and the official QFT ELISA, respectively. The IP-10 mRNA assay provided high specificity (98%), sensitivity (80%), and area under the concentration-time curve (AUC) (0.97); however, the QFT assay provided a higher overall diagnostic potential, with specificity of 100%, sensitivity of 90%, and AUC of 0.99. The IP-10 protein assay performed on par with the QFT assay, with specificity of 98%, sensitivity of 87%, and AUC of 0.98. We have provided proof of high technical performance of a molecular assay detecting IP-10 mRNA expression. As a diagnostic tool, this assay would gain from further optimization, especially on the kinetics of IP-10 mRNA expression.

AB - Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 100-fold-higher expression level than IFN-γ, allowing for different analysis platforms, including molecular detection. The PCR technique is already an integrated tool in most TB laboratories and, thus, an obvious platform to turn to. In this case-control study, we investigated the diagnostic sensitivity and specificity of a molecular assay detecting IP-10 mRNA expression following antigen stimulation of a blood sample. We included 89 TB patients and 99 healthy controls. Blood was drawn in QuantiFeron-TB gold in-tube (QFT) assay tubes. Eight hours poststimulation, IP-10 mRNA expression was analyzed, and 20 h poststimulation, IP-10 and IFN-γ protein plasma levels were analyzed using an in-house IP-10 enzyme-linked immunosorbent assay (ELISA) and the official QFT ELISA, respectively. The IP-10 mRNA assay provided high specificity (98%), sensitivity (80%), and area under the concentration-time curve (AUC) (0.97); however, the QFT assay provided a higher overall diagnostic potential, with specificity of 100%, sensitivity of 90%, and AUC of 0.99. The IP-10 protein assay performed on par with the QFT assay, with specificity of 98%, sensitivity of 87%, and AUC of 0.98. We have provided proof of high technical performance of a molecular assay detecting IP-10 mRNA expression. As a diagnostic tool, this assay would gain from further optimization, especially on the kinetics of IP-10 mRNA expression.

KW - IP-10

KW - diagnosis

KW - mRNA

KW - qPCR

KW - tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=85091585963&partnerID=8YFLogxK

U2 - 10.1128/JCM.00848-20

DO - 10.1128/JCM.00848-20

M3 - Journal article

VL - 58

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 10

ER -

ID: 60588456