TY - JOUR
T1 - Development of an Experimental Ex Vivo Wound Model to Evaluate Antimicrobial Efficacy of Topical Formulations
AU - Andersson, Madelene Å
AU - Madsen, Lone Bruhn
AU - Schmidtchen, Artur
AU - Puthia, Manoj
PY - 2021/5/10
Y1 - 2021/5/10
N2 - Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and reproducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial effects. Both bacterial strains successfully infected the wound surface, as well as deeper regions of the tissue. Quantification of viable bacteria on the wound surface and in the tissue, longitudinal measurements of bioluminescence, fluorescence microscopy, and scanning electron microscopy were used to confirm the effects of antibacterial treatments. Furthermore, we show that biofilms are formed on the wound surface, indicating that the demonstrated method mirrors typical in vivo infections.
AB - Wound infections are considered a major cause for wound-associated morbidity. There is a high demand for alternative, robust, and affordable methods that can provide relatable and reproducible results when testing topical treatments, both in research and in the pharmaceutical industry. Here we present an ex vivo wound infection model using porcine skin and a burn wounding method, allowing for the efficacy evaluation of topical antimicrobial formulations. Utilizing this model, we demonstrate the potential of topical treatments after infecting the wounds with clinically significant bacteria, P. aeruginosa and S. aureus. We show that the method is compatible with several analytical tools used to analyze infection and antimicrobial effects. Both bacterial strains successfully infected the wound surface, as well as deeper regions of the tissue. Quantification of viable bacteria on the wound surface and in the tissue, longitudinal measurements of bioluminescence, fluorescence microscopy, and scanning electron microscopy were used to confirm the effects of antibacterial treatments. Furthermore, we show that biofilms are formed on the wound surface, indicating that the demonstrated method mirrors typical in vivo infections.
KW - Administration, Topical
KW - Animals
KW - Anti-Bacterial Agents/chemistry
KW - Anti-Infective Agents/pharmacology
KW - Biofilms/drug effects
KW - Burns/drug therapy
KW - Disease Models, Animal
KW - Drug Compounding/methods
KW - Humans
KW - Pseudomonas Infections/drug therapy
KW - Pseudomonas aeruginosa/drug effects
KW - Staphylococcal Infections/drug therapy
KW - Staphylococcus aureus/drug effects
KW - Swine
KW - Wound Healing/drug effects
KW - Wound Infection/drug therapy
UR - http://www.scopus.com/inward/record.url?scp=85105458936&partnerID=8YFLogxK
U2 - 10.3390/ijms22095045
DO - 10.3390/ijms22095045
M3 - Journal article
C2 - 34068733
SN - 1661-6596
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
M1 - 5045
ER -