TY - JOUR
T1 - Development of a PCR assay for rapid and accurate detection of an emerging vanB Enterococcus faecium clone in the Capital Region of Denmark
AU - Knudsen, Maja Johanne Søndergaard
AU - Barker Jensen, Christel
AU - Jørgensen, Rikke Lind
AU - Petersen, Andreas Munk
AU - Qvist Kristiansen, Gitte
AU - Lisby, Jan Gorm
AU - Worning, Peder
AU - Westh, Henrik
AU - Pinholt, Mette
N1 - © The Author(s) 2024. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
PY - 2024/11/7
Y1 - 2024/11/7
N2 - OBJECTIVES: To develop and validate a real-time PCR assay detecting the sequence bridging Tn1549 and the Enterococcus faecium chromosome in the emerging vanB vancomycin-resistant E. faecium (VREfm) clone (ST80/CT2406).METHODS: The Tn1549 insertion site was determined on routinely sequenced VREfm isolates. The outer boundaries of Tn1549 and adjoining host bacterial sequences were determined using a BLAST search in the silent information regulator gene sir2. Next, the primers and probe were developed, targeting the sequence bridging Tn1549 and the E. faecium chromosome. Finally, the PCR assay was validated on well-characterized strains and prospectively performed on rectal screening samples submitted to our laboratory.RESULTS AND CONCLUSIONS: The PCR assay proved to be accurate and provide rapid diagnosis of the emerging vanB VREfm in rectal screening samples.
AB - OBJECTIVES: To develop and validate a real-time PCR assay detecting the sequence bridging Tn1549 and the Enterococcus faecium chromosome in the emerging vanB vancomycin-resistant E. faecium (VREfm) clone (ST80/CT2406).METHODS: The Tn1549 insertion site was determined on routinely sequenced VREfm isolates. The outer boundaries of Tn1549 and adjoining host bacterial sequences were determined using a BLAST search in the silent information regulator gene sir2. Next, the primers and probe were developed, targeting the sequence bridging Tn1549 and the E. faecium chromosome. Finally, the PCR assay was validated on well-characterized strains and prospectively performed on rectal screening samples submitted to our laboratory.RESULTS AND CONCLUSIONS: The PCR assay proved to be accurate and provide rapid diagnosis of the emerging vanB VREfm in rectal screening samples.
UR - http://www.scopus.com/inward/record.url?scp=85208534253&partnerID=8YFLogxK
U2 - 10.1093/jacamr/dlae180
DO - 10.1093/jacamr/dlae180
M3 - Journal article
C2 - 39512360
SN - 2632-1823
VL - 6
SP - dlae180
JO - JAC-antimicrobial resistance
JF - JAC-antimicrobial resistance
IS - 6
M1 - dlae180
ER -