Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Gender-partitioned patient medians of serum albumin requested by general practitioners for the assessment of analytical stability

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Individual values of antineutrophil cytoplasmic antibodies do not correspond between antigen-specific assays

    Publikation: Bidrag til tidsskriftLetterForskningpeer review

  3. Precision of nonfasting lipid profiles should focus on clinical relevance rather than necessarily obtaining the least variation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Characterisation and localisation of the endocannabinoid system components in the adult human testis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Development and Interlaboratory Validation of Two Fast UPLC-MS-MS Methods Determining Urinary Bisphenols, Parabens and Phthalates

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Possible link between FSH and RANKL release from adipocytes in men with impaired gonadal function including Klinefelter syndrome

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Characterization of Human Adrenal Steroidogenesis during Fetal Development

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: The circulating level of the peptide hormone insulin-like factor 3 (INSL3) is a promising diagnostic marker reflecting Leydig cell function in the male. Few commercial immunoassays of varying quality exist. Therefore, we decided to develop and validate a precise method for quantification of INSL3 by mass spectrometry.

METHODS: We developed an assay in which the INSL3 A-chain is released from the INSL3 A-B heterodimer by chemical reduction and alkylation. The alkylated INSL3 A-chain is quantitated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), as substitute for serum INSL3. The method was compared to a validated and sensitive in-house serum INSL3 immunoassay using 97 serum samples from 12 healthy boys during pubertal transition. Adult levels were determined based on sera from 72 adult healthy males aged 18-40 years.

RESULTS: An LC-MS/MS assay with limit of detection and limit of quantification (LOQ) of 0.06 and 0.15 ng/mL, respectively, and intra-assay CVs <9% in the relevant ranges was obtained. The LC-MS/MS compared well with the in-house immunoassay (Deming regression slope: 1.28; Pearson correlation: R=0.86). INSL3 concentrations increased with pubertal maturation in healthy boys. INSL3 concentrations were above the LOQ in all samples from the adult men. The mean (±2 SD range)for serum INSL3 concentrations in the adult men was 2.2 (0.5-3.9) ng/mL.

CONCLUSIONS: We have developed a robust and sensitive method suitable for quantitation of serum INSL3 in a clinical setting using LC-MS/MS instrumentation available in modern clinical laboratories. The method paves the way for future studies into the clinical role of serum INSL3 measurements.

OriginalsprogEngelsk
TidsskriftClinical Chemistry and Laboratory Medicine
Vol/bind56
Udgave nummer11
Sider (fra-til)1913-20
ISSN1434-6621
DOI
StatusUdgivet - 2018

ID: 54666727