Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

S A Koppes; P Kemperman; I van Tilburg; F Calkoen-Kwa; K A Engebretsen; G J Puppels; P J Caspers; S Kezic

doi:10.1080/1354750X.2016.1256428

Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

S A Koppes
, P Kemperman
, I van Tilburg
, F Calkoen-Kwa
, K A Engebretsen
, G J Puppels
, P J Caspers
, S Kezic

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34 Citationer (Scopus)

Abstract

BACKGROUND: Natural moisturizing factors (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.

RESULTS: Measured NMF values were significantly correlated (R2 = 0.61; P < 0.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicate robustness and good reproducibility of both methods.

CONCLUSION: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

Originalsprog	Engelsk
Tidsskrift	Biomarkers
Vol/bind	22
Udgave nummer	6
Sider (fra-til)	502-507
ISSN	1354-750X
DOI	https://doi.org/10.1080/1354750X.2016.1256428
Status	Udgivet - 18 aug. 2017

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10.1080/1354750X.2016.1256428

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title = "Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC",

abstract = "BACKGROUND: Natural moisturizing factors (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.RESULTS: Measured NMF values were significantly correlated (R2 = 0.61; P < 0.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicate robustness and good reproducibility of both methods.CONCLUSION: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.",

author = "Koppes, \{S A\} and P Kemperman and \{van Tilburg\}, I and F Calkoen-Kwa and Engebretsen, \{K A\} and Puppels, \{G J\} and Caspers, \{P J\} and S Kezic",

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doi = "10.1080/1354750X.2016.1256428",

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TY - JOUR

T1 - Determination of natural moisturizing factors in the skin

T2 - Raman microspectroscopy versus HPLC

AU - Koppes, S A

AU - Kemperman, P

AU - van Tilburg, I

AU - Calkoen-Kwa, F

AU - Engebretsen, K A

AU - Puppels, G J

AU - Caspers, P J

AU - Kezic, S

PY - 2017/8/18

Y1 - 2017/8/18

N2 - BACKGROUND: Natural moisturizing factors (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.RESULTS: Measured NMF values were significantly correlated (R2 = 0.61; P < 0.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicate robustness and good reproducibility of both methods.CONCLUSION: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

AB - BACKGROUND: Natural moisturizing factors (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.RESULTS: Measured NMF values were significantly correlated (R2 = 0.61; P < 0.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicate robustness and good reproducibility of both methods.CONCLUSION: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

U2 - 10.1080/1354750X.2016.1256428

DO - 10.1080/1354750X.2016.1256428

M3 - Journal article

C2 - 27805415

SN - 1354-750X

VL - 22

SP - 502

EP - 507

JO - Biomarkers

JF - Biomarkers

IS - 6

ER -

Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Abstract

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