Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection

H R Christensen; H R Angelo; K Skajaa

doi:10.1016/0378-4347(92)80113-5

Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection

H R Christensen, H R Angelo, K Skajaa

Klinisk Farmakologisk Afdeling BFH

11 Citationer (Scopus)

Abstract

A relatively simple, sensitive and precise gas chromatographic method for the determination of isradipine, a calcium antagonist of the dihydropyridine type, and its main metabolite in serum is described. Using a one-step extraction procedure, a wide-bore column and a nitrogen-phosphorus detector, a limit of quantitation of 0.5 and 2.0 nM for isradipine and the metabolite was found. No interferences from several drugs were observed. The method was successfully used in a pharmacokinetic study in hypertensive women during pregnancy.

Originalsprog	Engelsk
Tidsskrift	Journal of Chromatography
Vol/bind	574
Udgave nummer	1
Sider (fra-til)	161-5
Antal sider	5
ISSN	0021-9673
DOI	https://doi.org/10.1016/0378-4347(92)80113-5
Status	Udgivet - 7 feb. 1992

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10.1016/0378-4347(92)80113-5

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title = "Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection",

abstract = "A relatively simple, sensitive and precise gas chromatographic method for the determination of isradipine, a calcium antagonist of the dihydropyridine type, and its main metabolite in serum is described. Using a one-step extraction procedure, a wide-bore column and a nitrogen-phosphorus detector, a limit of quantitation of 0.5 and 2.0 nM for isradipine and the metabolite was found. No interferences from several drugs were observed. The method was successfully used in a pharmacokinetic study in hypertensive women during pregnancy.",

keywords = "Calcium Channel Blockers/blood, Chromatography, Gas, Dihydropyridines/blood, Humans, Isradipine, Nitrogen, Pyridines/metabolism, Reproducibility of Results",

author = "Christensen, {H R} and Angelo, {H R} and K Skajaa",

year = "1992",

month = feb,

day = "7",

doi = "10.1016/0378-4347(92)80113-5",

language = "English",

volume = "574",

pages = "161--5",

journal = "Journal of Chromatography",

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TY - JOUR

T1 - Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection

AU - Christensen, H R

AU - Angelo, H R

AU - Skajaa, K

PY - 1992/2/7

Y1 - 1992/2/7

N2 - A relatively simple, sensitive and precise gas chromatographic method for the determination of isradipine, a calcium antagonist of the dihydropyridine type, and its main metabolite in serum is described. Using a one-step extraction procedure, a wide-bore column and a nitrogen-phosphorus detector, a limit of quantitation of 0.5 and 2.0 nM for isradipine and the metabolite was found. No interferences from several drugs were observed. The method was successfully used in a pharmacokinetic study in hypertensive women during pregnancy.

AB - A relatively simple, sensitive and precise gas chromatographic method for the determination of isradipine, a calcium antagonist of the dihydropyridine type, and its main metabolite in serum is described. Using a one-step extraction procedure, a wide-bore column and a nitrogen-phosphorus detector, a limit of quantitation of 0.5 and 2.0 nM for isradipine and the metabolite was found. No interferences from several drugs were observed. The method was successfully used in a pharmacokinetic study in hypertensive women during pregnancy.

KW - Calcium Channel Blockers/blood

KW - Chromatography, Gas

KW - Dihydropyridines/blood

KW - Humans

KW - Isradipine

KW - Nitrogen

KW - Pyridines/metabolism

KW - Reproducibility of Results

U2 - 10.1016/0378-4347(92)80113-5

DO - 10.1016/0378-4347(92)80113-5

M3 - Journal article

C2 - 1385820

SN - 0021-9673

VL - 574

SP - 161

EP - 165

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1

ER -

Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection

Abstract

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