Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis. Results from the ABIRISK prospective cohort study

Poul Erik H Jensen, Clemens Warnke, Kathleen Ingenhoven, Luca Piccoli, Marcia Gasis, Christina Hermanrud, Blanca M Fernandez-Rodriguez, Malin Ryner, Daniel Kramer, Jenny Link, Ryan Ramanujam, Michael Auer, Dorothea Buck, Verena Grummel, Elisa Bertotti, Nicolas Fissolo, Begoña Oliver-Martos, Petra Nytrova, Michael Khalil, Michael GugerSandra Rathmaier, Claudia Sievers-Stober, Raija L P Lindberg, Signe Hässler, Delphine Bachelet, Orhan Aktas, Naoimh Donnellan, Andy Lawton, Bernhard Hemmer, Eva Kubala Havrdova, Bernd Kieseier, Hans-Peter Hartung, Manuel Comabella, Xavier Montalban, Tobias Derfuss, Finn Sellebjerg, Pierre Dönnes, Marc Pallardy, Sebastian Spindeldreher, Philippe Broët, Florian Deisenhammer, Anna Fogdell-Hahn, Per Soelberg Sorensen, ABIRISK Consortium

Abstract

Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) against interferon-beta (IFN-β) in patients with multiple sclerosis. Serum samples were tested from patients enrolled in a prospective study of 18 months. In contrast to the ELISA, when IFN-β-specific rabbit polyclonal and human monoclonal antibodies were tested, the bioassay was the more sensitive to detect IFN-β ADA in patients' sera. For clinical samples, selection of method of ELISA should be evaluated prior to the use of a multi-tiered approach. A titer threshold value is reported that may be used as a predictor for persistently positive neutralizing ADA.

OriginalsprogEngelsk
TidsskriftJournal of Neuroimmunology
Vol/bind326
Sider (fra-til)19-27
Antal sider9
ISSN0165-5728
DOI
StatusUdgivet - 15 jan. 2019

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