Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Describing the fecal metabolome in cryogenically collected samples from healthy participants

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Glycemic control and use of glucose-lowering medications in hospital-admitted type 2 diabetes patients over 80 years

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Lipidomic profiles, lipid trajectories and clinical biomarkers in female elite endurance athletes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Author Correction: Characterisation and localisation of the endocannabinoid system components in the adult human testis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. A prospective three-year follow-up study on the clinical significance of anti-neuronal antibodies in acute psychiatric disorders

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Leptin, adiponectin, and their ratio as markers of insulin resistance and cardiometabolic risk in childhood obesity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Blood Metabolite Signatures of Metabolic Syndrome in Two Cross-Cultural Older Adult Cohorts

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Lipidomic profiles, lipid trajectories and clinical biomarkers in female elite endurance athletes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Relation of cardiac adipose tissue to coronary calcification and myocardial microvascular function in type 1 and type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The chemical composition of feces plays an important role in human metabolism. Metabolomics and lipidomics are valuable tools for screening the metabolite composition in feces. Here we set out to describe fecal metabolite composition in healthy participants in frozen stools. Frozen stool samples were collected from 10 healthy volunteers and cryogenically drilled in four areas along the specimen. Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatography and time of flight mass spectrometry. Lipids were detected using ultra high-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. 2326 metabolic features were detected. Out of a total of 298 metabolites that were annotated we report here 185 that showed a technical variation of x < 30%. These metabolites included amino acids, fatty acid derivatives, carboxylic acids and phenolic compounds. Lipids predominantly belonged to the groups of diacylglycerols, triacylglycerols and ceramides. Metabolites varied between sampling areas, some were broadly homogeneous, others varied 80%. A LASSO-computed network using metabolites present in all areas showed two main clusters describing the system, DAG lipids and phenyllactic acid. In feces from healthy participants, the main groups detected were phenolic compounds, ceramides, diacylglycerols and triacylglycerols.

OriginalsprogEngelsk
Artikelnummer885
TidsskriftScientific Reports
Vol/bind10
ISSN2045-2322
DOI
StatusUdgivet - 21 jan. 2020

ID: 59247793