Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Decreased spontaneous activity in AMPK α2 muscle specific kinase dead mice is not caused by changes in brain dopamine metabolism

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Novel GLP-1/GLP-2 co-agonists display marked effects on gut volume and improves glycemic control in mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The glucagon-like peptide 1 (GLP-1) receptor agonist exendin-4 reduces cocaine self-administration in mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Stress and reward: long term cortisol exposure predicts the strength of sexual preference

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Electro convulsive therapy: Modification of its effect on the autonomic nervous system using anti-cholinergic drugs

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Differential plastic changes in synthesis and binding in the mouse somatostatin system after electroconvulsive stimulation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. PICK1-Deficient Mice Exhibit Impaired Response to Cocaine and Dysregulated Dopamine Homeostasis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Cerebral serotonin release correlates with [11C]AZ10419369 PET measures of 5-HT1B receptor binding in the pig brain

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

It is well known that physical activity has several health benefits, yet many people do not exercise. Dopamine levels in the striatum of the brain are thought to be important for the motivation to exercise. Conversely, we hypothesized that muscle quality can affect the motivation to exercise through alterations of the brain dopamine levels specifically in the striatal region. To test this hypothesis, transgenic mice overexpressing an inactivatable dominant negative α2 AMPK construct (AMPK α2 KD) in muscles and littermate wildtype (WT) mice were tested. AMPK α2 KD mice have impaired running capacity and display reduced voluntary wheel running activity. Striatal content of dopamine and its metabolites were measured under basal physiological conditions and after cocaine-induced dopamine efflux from the ventral striatum by in vivo microdialysis. Moreover, cocaine-induced locomotor activity was tested in an open field test. Furthermore, we investigated maximal running capacity and voluntary running over a period of 19days. AMPK α2 KD mice ran 30% less in daily distance compared to WT. Furthermore, AMPK α2 KD mice showed significantly decreased locomotor activity in the open field test compared to WT when treated with saline or cocaine, respectively, but the increase induced by cocaine was similar in AMPK α2 KD and WT mice. The efflux of dopamine in ventral striatum after cocaine treatment increased similarly by 2.5-fold in the two genotypes, and basal levels of dopamine and its metabolites DOPAC and HVA were also similar between genotypes. These findings show that decreased AMPK activity in muscle leads to decreased voluntary activity which is not due to secondary abnormalities in dopamine levels in the ventral striatum or sensitivity to cocaine. Thus, decreased voluntary activity in AMPK muscle deficient mice is most likely unrelated to regulation of brain dopamine content and metabolism.

OriginalsprogEngelsk
TidsskriftPhysiology and Behavior
Vol/bind164
Udgave nummerPt A
Sider (fra-til)300-5
Antal sider6
ISSN0031-9384
DOI
StatusUdgivet - 1 okt. 2016

ID: 49663227