Harvard
Greve, T, Clasen-Linde, E, Andersen, MT, Andersen, MK, Sørensen, SD, Rosendahl, M, Ralfkiær, E & Yding Andersen, C 2012, '
Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells'
Blood, bind 120, nr. 22, s. 4311-4316.
https://doi.org/10.1182/blood-2012-01-403022
APA
Greve, T., Clasen-Linde, E., Andersen, M. T., Andersen, M. K., Sørensen, S. D., Rosendahl, M., ... Yding Andersen, C. (2012).
Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells.
Blood,
120(22), 4311-4316.
https://doi.org/10.1182/blood-2012-01-403022
CBE
MLA
Vancouver
Author
Bibtex
@article{79dd1743a86f4d44b828974a5c843cc3,
title = "Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells",
abstract = "Some women suffering from leukemia require bone marrow transplantation to be cured. Bone marrow transplantation is associated with a high risk of sterility and some patients are offered fertility preservation by cryopreservation of ovarian cortex. Transplantation of ovarian cortex to women cured of leukemia, who became menopausal, is currently not performed due to the risk of introducing the disease. In this study, individual pieces of ovarian cortex intended for reimplantation from 25 patients with leukemia were transplanted to each of 25 nude mice for 20 weeks. The ovarian cortex was examined before and after transplantation by histology and immunohistochemistry, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) (in the seven patients with a known marker). Seventeen patients had ovarian cortex retrieved when they were in complete remission. Before transplantation four of seven pieces (two from patients in complete remission) of ovarian cortex had a positive RT-qPCR. After transplantation, none of the mice revealed any sign of disease neither in the pieces of ovarian cortex transplanted nor in any of the murine organs evaluated. Thus, the ovaries from patients in complete remission do not appear to contain viable malignant cells contrasting ovarian tissue retrieved prior to treatment.",
author = "Tine Greve and Erik Clasen-Linde and Andersen, {Morten T} and Andersen, {Mette K} and S{\o}rensen, {Stine D} and Mikkel Rosendahl and Elisabeth Ralfki{\ae}r and {Yding Andersen}, Claus",
year = "2012",
doi = "10.1182/blood-2012-01-403022",
language = "English",
volume = "120",
pages = "4311--4316",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "22",
}
RIS
TY - JOUR
T1 - Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells
AU - Greve, Tine
AU - Clasen-Linde, Erik
AU - Andersen, Morten T
AU - Andersen, Mette K
AU - Sørensen, Stine D
AU - Rosendahl, Mikkel
AU - Ralfkiær, Elisabeth
AU - Yding Andersen, Claus
PY - 2012
Y1 - 2012
N2 - Some women suffering from leukemia require bone marrow transplantation to be cured. Bone marrow transplantation is associated with a high risk of sterility and some patients are offered fertility preservation by cryopreservation of ovarian cortex. Transplantation of ovarian cortex to women cured of leukemia, who became menopausal, is currently not performed due to the risk of introducing the disease. In this study, individual pieces of ovarian cortex intended for reimplantation from 25 patients with leukemia were transplanted to each of 25 nude mice for 20 weeks. The ovarian cortex was examined before and after transplantation by histology and immunohistochemistry, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) (in the seven patients with a known marker). Seventeen patients had ovarian cortex retrieved when they were in complete remission. Before transplantation four of seven pieces (two from patients in complete remission) of ovarian cortex had a positive RT-qPCR. After transplantation, none of the mice revealed any sign of disease neither in the pieces of ovarian cortex transplanted nor in any of the murine organs evaluated. Thus, the ovaries from patients in complete remission do not appear to contain viable malignant cells contrasting ovarian tissue retrieved prior to treatment.
AB - Some women suffering from leukemia require bone marrow transplantation to be cured. Bone marrow transplantation is associated with a high risk of sterility and some patients are offered fertility preservation by cryopreservation of ovarian cortex. Transplantation of ovarian cortex to women cured of leukemia, who became menopausal, is currently not performed due to the risk of introducing the disease. In this study, individual pieces of ovarian cortex intended for reimplantation from 25 patients with leukemia were transplanted to each of 25 nude mice for 20 weeks. The ovarian cortex was examined before and after transplantation by histology and immunohistochemistry, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) (in the seven patients with a known marker). Seventeen patients had ovarian cortex retrieved when they were in complete remission. Before transplantation four of seven pieces (two from patients in complete remission) of ovarian cortex had a positive RT-qPCR. After transplantation, none of the mice revealed any sign of disease neither in the pieces of ovarian cortex transplanted nor in any of the murine organs evaluated. Thus, the ovaries from patients in complete remission do not appear to contain viable malignant cells contrasting ovarian tissue retrieved prior to treatment.
U2 - 10.1182/blood-2012-01-403022
DO - 10.1182/blood-2012-01-403022
M3 - Journal article
VL - 120
SP - 4311
EP - 4316
JO - Blood
JF - Blood
SN - 0006-4971
IS - 22
ER -