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Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Futures and fears in the freezer: Danish women's experiences with ovarian tissue cryopreservation and transplantation

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  1. Expression of the Insulin-like Growth Factor System in First- and Second-Trimester Human Embryonic and Fetal Gonads

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Futures and fears in the freezer: Danish women's experiences with ovarian tissue cryopreservation and transplantation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Shortcomings of an unphysiological triggering of oocyte maturation using human chorionic gonadotropin

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  4. Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex

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This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological evaluation of mouse and human ovarian tissue after freezing with four different combinations of cryoprotectants. Viability was confirmed by transplantation of frozen-thawed human ovarian tissue (n=49) to oophorectomized Nude mice. Viability after transport of fresh tissue 4-5h prior to freezing had previously been validated. Overnight transport of fresh ovarian tissue prior to cryopreservation was evaluated when human ovarian tissue was kept on ice for 20h and then cryopreserved. The thawed ovarian tissue was transplanted to an oophorectomized Nude mouse and histology confirmed viability. In Denmark 12 women have received a total of 18 autotransplantations of ovarian tissue. All women resumed ovarian function and three healthy babies were born to two women. In both women, the tissue was transported on ice for 4-5h prior to cryopreservation. Ovarian tissue cryopreservation is an important method for fertility preservation; however, before applying the method clinically, each laboratory should perform thorough validation of their technique.
OriginalsprogEngelsk
TidsskriftReproductive BioMedicine Online
Vol/bind22
Udgave nummer2
Sider (fra-til)162-71
Antal sider10
ISSN1472-6483
DOI
StatusUdgivet - 1 feb. 2011

ID: 31040797