Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Cryopreservation and Revival of Human Mesenchymal Stromal Cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Analysis of Mass Cytometry Data

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Inflammation, non-endothelial dependent coronary microvascular function and diastolic function-Are they linked?

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Prognosis and Reclassification by YKL-40 in Stable Coronary Artery Disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Cell-based therapy is a promising and innovative new treatment for different degenerative and autoimmune diseases, and mesenchymal stromal cells (MSCs) from the bone marrow have demonstrated great therapeutic potential due to their immunosuppressive and regenerative capacities.The establishment of methods for large-scale expansion of clinical-grade MSCs in vitro has paved the way for their therapeutic use in clinical trials. However, the clinical application of MSCs also requires cryopreservation and banking of the cell products. To preserve autologous or allogeneic MSCs for future clinical applications, a reliable and effective cryopreservation method is required.Developing a successful cryopreservation protocol for clinical stem cell products, cryopreservation media, cryoprotectant agents (CPAs), the freezing container, the freezing temperature, and the cooling and warming rate are all aspects which should be considered.A major challenge is the selection of a suitable cryoprotectant which is able to penetrate the cells and yet has low toxicity.This chapter focuses on recent technological developments relevant for the cryopreservation of MSCs using the most commonly used cryopreservation medium containing DMSO and animal serum or human-derived products for research use and the animal protein-free cryopreservation media CryoStor (BioLife Solutions) for clinical use.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1416
Sider (fra-til)357-74
Antal sider18
ISSN1064-3745
DOI
StatusUdgivet - 2016

ID: 49799753