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Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests

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Ndiaye, Magatte ; Sow, Doudou ; Nag, Sidsel ; Sylla, Khadime ; Tine, Roger Clement ; Ndiaye, Jean Louis ; Lo, Aminata Collé ; Gaye, Oumar ; Faye, Babacar ; Alifrangis, Michael. / Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests. I: The American journal of tropical medicine and hygiene. 2017 ; Bind 97, Nr. 5. s. 1593-1596.

Bibtex

@article{0f68e898bcd14dadbd5f1cc1c035eb1f,
title = "Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests",
abstract = "In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers ofPlasmodium falciparumparasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regardingPfcrtgene polymorphisms at codon 72-76. Overall, a high but varied prevalence (> 50{\%}) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75{\%}) compared with the southern part of the country (59{\%}). With caution, the study provides a proof of concept that reuse of discardedP. falciparumpositive RDTs can be applied in large-scale surveillance of antimalarial drug resistance.",
keywords = "Antimalarials, Diagnostic Tests, Routine, Drug Resistance, Genetic Markers, Haplotypes, Humans, Malaria, Falciparum, Membrane Transport Proteins, Pilot Projects, Plasmodium falciparum, Polymorphism, Single Nucleotide, Population Surveillance, Prevalence, Protozoan Proteins, Senegal, Journal Article, Multicenter Study",
author = "Magatte Ndiaye and Doudou Sow and Sidsel Nag and Khadime Sylla and Tine, {Roger Clement} and Ndiaye, {Jean Louis} and Lo, {Aminata Coll{\'e}} and Oumar Gaye and Babacar Faye and Michael Alifrangis",
year = "2017",
month = "11",
doi = "10.4269/ajtmh.17-0021",
language = "English",
volume = "97",
pages = "1593--1596",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "5",

}

RIS

TY - JOUR

T1 - Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests

AU - Ndiaye, Magatte

AU - Sow, Doudou

AU - Nag, Sidsel

AU - Sylla, Khadime

AU - Tine, Roger Clement

AU - Ndiaye, Jean Louis

AU - Lo, Aminata Collé

AU - Gaye, Oumar

AU - Faye, Babacar

AU - Alifrangis, Michael

PY - 2017/11

Y1 - 2017/11

N2 - In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers ofPlasmodium falciparumparasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regardingPfcrtgene polymorphisms at codon 72-76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discardedP. falciparumpositive RDTs can be applied in large-scale surveillance of antimalarial drug resistance.

AB - In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers ofPlasmodium falciparumparasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regardingPfcrtgene polymorphisms at codon 72-76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discardedP. falciparumpositive RDTs can be applied in large-scale surveillance of antimalarial drug resistance.

KW - Antimalarials

KW - Diagnostic Tests, Routine

KW - Drug Resistance

KW - Genetic Markers

KW - Haplotypes

KW - Humans

KW - Malaria, Falciparum

KW - Membrane Transport Proteins

KW - Pilot Projects

KW - Plasmodium falciparum

KW - Polymorphism, Single Nucleotide

KW - Population Surveillance

KW - Prevalence

KW - Protozoan Proteins

KW - Senegal

KW - Journal Article

KW - Multicenter Study

U2 - 10.4269/ajtmh.17-0021

DO - 10.4269/ajtmh.17-0021

M3 - Journal article

VL - 97

SP - 1593

EP - 1596

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 5

ER -

ID: 52820807