Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Slott Jensen, Marie Louise ; Skov, Marianne Nielsine ; Kristiansen, Helle Pries ; Toft, Annette ; Lundgaard, Hanne ; Gumpert, Heidi ; Westh, Henrik ; Holm, Anette ; Kolmos, Hans Jørn ; Kemp, Michael. / Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak. I: The Journal of hospital infection. 2020 ; Bind 104, Nr. 4. s. 574-581.

Bibtex

@article{e64804de31274141ad8968c5ba5b70a9,
title = "Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak",
abstract = "Background: Livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 may be transmitted and cause morbidity and mortality in hospitals. The economic cost of stopping hospital transmission of LA-MRSA CC398 is poorly described. Early detection of transmission may limit the extent of the intervention. Aim: To evaluate core genome multi-locus sequence typing (cgMLST) for detecting transmission chains and to estimate the costs for interventions to prevent further spread after discovery of hospital transmission of LA-MRSA CC398. Methods: Five patients were involved in two episodes of transmission of LA-MRSA CC398 in a hospital. Standard interventions including MRSA screening of patients and healthcare workers were initiated. Whole genome sequences of the five isolates and 17 epidemiologically unrelated MRSA CC398 isolates from other hospitalized patients were analysed by single nucleotide polymorphism (SNP) comparisons and cgMLST. The economic costs of constraining transmission were calculated from relevant sources. Findings: The five isolates suspected to be involved in hospital transmission clustered with ≤2 SNPs in the draft genome sequences with some distance to other isolates. cgMLST allocated the five isolates to the same type, which was different from all but two of the sporadic isolates. Furthermore, cgMLST separated the five transmission isolates from all other isolates. The economic costs of the outbreak interventions exceeded €11,000 per patient. Conclusion: LA-MRSA CC398 is transmittable in hospitals, and intervention against transmission may reach considerable costs. cgMLST is useful in surveillance of hospital transmission of LA-MRSA.",
keywords = "cgMLST, Economic costs, Hospital outbreak, Livestock-associated MRSA, MRSA CC398, Whole genome sequencing",
author = "{Slott Jensen}, {Marie Louise} and Skov, {Marianne Nielsine} and Kristiansen, {Helle Pries} and Annette Toft and Hanne Lundgaard and Heidi Gumpert and Henrik Westh and Anette Holm and Kolmos, {Hans J{\o}rn} and Michael Kemp",
note = "Copyright {\circledC} 2019 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.",
year = "2020",
month = "4",
doi = "10.1016/j.jhin.2019.12.009",
language = "English",
volume = "104",
pages = "574--581",
journal = "Journal of Hospital Infection",
issn = "0195-6701",
publisher = "W.B./Saunders Co. Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Core genome multi-locus sequence typing as an essential tool in a high-cost livestock-associated meticillin-resistant Staphylococcus aureus CC398 hospital outbreak

AU - Slott Jensen, Marie Louise

AU - Skov, Marianne Nielsine

AU - Kristiansen, Helle Pries

AU - Toft, Annette

AU - Lundgaard, Hanne

AU - Gumpert, Heidi

AU - Westh, Henrik

AU - Holm, Anette

AU - Kolmos, Hans Jørn

AU - Kemp, Michael

N1 - Copyright © 2019 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

PY - 2020/4

Y1 - 2020/4

N2 - Background: Livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 may be transmitted and cause morbidity and mortality in hospitals. The economic cost of stopping hospital transmission of LA-MRSA CC398 is poorly described. Early detection of transmission may limit the extent of the intervention. Aim: To evaluate core genome multi-locus sequence typing (cgMLST) for detecting transmission chains and to estimate the costs for interventions to prevent further spread after discovery of hospital transmission of LA-MRSA CC398. Methods: Five patients were involved in two episodes of transmission of LA-MRSA CC398 in a hospital. Standard interventions including MRSA screening of patients and healthcare workers were initiated. Whole genome sequences of the five isolates and 17 epidemiologically unrelated MRSA CC398 isolates from other hospitalized patients were analysed by single nucleotide polymorphism (SNP) comparisons and cgMLST. The economic costs of constraining transmission were calculated from relevant sources. Findings: The five isolates suspected to be involved in hospital transmission clustered with ≤2 SNPs in the draft genome sequences with some distance to other isolates. cgMLST allocated the five isolates to the same type, which was different from all but two of the sporadic isolates. Furthermore, cgMLST separated the five transmission isolates from all other isolates. The economic costs of the outbreak interventions exceeded €11,000 per patient. Conclusion: LA-MRSA CC398 is transmittable in hospitals, and intervention against transmission may reach considerable costs. cgMLST is useful in surveillance of hospital transmission of LA-MRSA.

AB - Background: Livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 may be transmitted and cause morbidity and mortality in hospitals. The economic cost of stopping hospital transmission of LA-MRSA CC398 is poorly described. Early detection of transmission may limit the extent of the intervention. Aim: To evaluate core genome multi-locus sequence typing (cgMLST) for detecting transmission chains and to estimate the costs for interventions to prevent further spread after discovery of hospital transmission of LA-MRSA CC398. Methods: Five patients were involved in two episodes of transmission of LA-MRSA CC398 in a hospital. Standard interventions including MRSA screening of patients and healthcare workers were initiated. Whole genome sequences of the five isolates and 17 epidemiologically unrelated MRSA CC398 isolates from other hospitalized patients were analysed by single nucleotide polymorphism (SNP) comparisons and cgMLST. The economic costs of constraining transmission were calculated from relevant sources. Findings: The five isolates suspected to be involved in hospital transmission clustered with ≤2 SNPs in the draft genome sequences with some distance to other isolates. cgMLST allocated the five isolates to the same type, which was different from all but two of the sporadic isolates. Furthermore, cgMLST separated the five transmission isolates from all other isolates. The economic costs of the outbreak interventions exceeded €11,000 per patient. Conclusion: LA-MRSA CC398 is transmittable in hospitals, and intervention against transmission may reach considerable costs. cgMLST is useful in surveillance of hospital transmission of LA-MRSA.

KW - cgMLST

KW - Economic costs

KW - Hospital outbreak

KW - Livestock-associated MRSA

KW - MRSA CC398

KW - Whole genome sequencing

UR - http://www.scopus.com/inward/record.url?scp=85078716296&partnerID=8YFLogxK

U2 - 10.1016/j.jhin.2019.12.009

DO - 10.1016/j.jhin.2019.12.009

M3 - Journal article

VL - 104

SP - 574

EP - 581

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

IS - 4

ER -

ID: 58720664