TY - JOUR
T1 - Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle
AU - Alsted, Thomas Junker
AU - Ploug, Thorkil
AU - Prats, Clara
AU - Serup, Annette Karen Lundbeck
AU - Høeg, Louise Dalgas
AU - Schjerling, Peter
AU - Holm, Cecilia
AU - Zimmermann, Robert
AU - Fledelius, Christian
AU - Galbo, Henrik
AU - Kiens, Bente
PY - 2013/10/15
Y1 - 2013/10/15
N2 - In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL knockout (HSL-KO) mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibres. To circumvent the problem with this contamination we analysed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (P
AB - In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL knockout (HSL-KO) mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibres. To circumvent the problem with this contamination we analysed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (P
U2 - 10.1113/jphysiol.2013.260794
DO - 10.1113/jphysiol.2013.260794
M3 - Journal article
C2 - 23878361
SN - 0022-3751
VL - 591
SP - 5141
EP - 5155
JO - The Journal of physiology
JF - The Journal of physiology
IS - Pt 20
ER -