Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo

Hans Peter M. Mortensen Lauritzen; Josef Brandauer; Peter Schjerling; Ho-Jin Koh; Jonas Thue Treebak; Michael F Hirshman; Henrik Galbo; Laurie J Goodyear

doi:10.2337/db12-1261

Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo

Hans Peter M. Mortensen Lauritzen, Josef Brandauer, Peter Schjerling, Ho-Jin Koh, Jonas Thue Treebak, Michael F Hirshman, Henrik Galbo, Laurie J Goodyear

Ortopædkirurgisk Afdeling M

58 Citationer (Scopus)

Abstract

Recent studies suggest that interleukin 6 (IL-6) is released from contracting skeletal muscles; however, the cellular origin, secretion kinetics, and signaling mechanisms regulating IL-6 secretion are unknown. To address these questions, we developed imaging methodology to study IL-6 in fixed mouse muscle fibers and in live animals in vivo. Using confocal imaging to visualize endogenous IL-6 protein in fixed muscle fibers, we found IL-6 in small vesicle structures distributed throughout the fibers under basal (resting) conditions. To determine the kinetics of IL-6 secretion, intact quadriceps muscles were transfected with enhanced green fluorescent protein (EGFP)-tagged IL-6 (IL-6-EGFP), and 5 days later anesthetized mice were imaged before and after muscle contractions in situ. Contractions decreased IL-6-EGFP-containing vesicles and protein by 62% (P

Originalsprog	Engelsk
Tidsskrift	Diabetes
Vol/bind	62
Udgave nummer	9
Sider (fra-til)	3081-92
Antal sider	12
ISSN	0012-1797
DOI	https://doi.org/10.2337/db12-1261
Status	Udgivet - sep. 2013

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10.2337/db12-1261

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title = "Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo",

abstract = "Recent studies suggest that interleukin 6 (IL-6) is released from contracting skeletal muscles; however, the cellular origin, secretion kinetics, and signaling mechanisms regulating IL-6 secretion are unknown. To address these questions, we developed imaging methodology to study IL-6 in fixed mouse muscle fibers and in live animals in vivo. Using confocal imaging to visualize endogenous IL-6 protein in fixed muscle fibers, we found IL-6 in small vesicle structures distributed throughout the fibers under basal (resting) conditions. To determine the kinetics of IL-6 secretion, intact quadriceps muscles were transfected with enhanced green fluorescent protein (EGFP)-tagged IL-6 (IL-6-EGFP), and 5 days later anesthetized mice were imaged before and after muscle contractions in situ. Contractions decreased IL-6-EGFP-containing vesicles and protein by 62% (P ",

keywords = "Aminoimidazole Carboxamide, Animals, Green Fluorescent Proteins, Interleukin-6, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Confocal, Muscle Contraction, Muscle Fibers, Skeletal, Ribonucleotides",

author = "Lauritzen, {Hans Peter M. Mortensen} and Josef Brandauer and Peter Schjerling and Ho-Jin Koh and Treebak, {Jonas Thue} and Hirshman, {Michael F} and Henrik Galbo and Goodyear, {Laurie J}",

year = "2013",

month = sep,

doi = "10.2337/db12-1261",

language = "English",

volume = "62",

pages = "3081--92",

journal = "Diabetes",

issn = "0012-1797",

publisher = "American Diabetes Association",

number = "9",

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TY - JOUR

T1 - Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo

AU - Lauritzen, Hans Peter M. Mortensen

AU - Brandauer, Josef

AU - Schjerling, Peter

AU - Koh, Ho-Jin

AU - Treebak, Jonas Thue

AU - Hirshman, Michael F

AU - Galbo, Henrik

AU - Goodyear, Laurie J

PY - 2013/9

Y1 - 2013/9

N2 - Recent studies suggest that interleukin 6 (IL-6) is released from contracting skeletal muscles; however, the cellular origin, secretion kinetics, and signaling mechanisms regulating IL-6 secretion are unknown. To address these questions, we developed imaging methodology to study IL-6 in fixed mouse muscle fibers and in live animals in vivo. Using confocal imaging to visualize endogenous IL-6 protein in fixed muscle fibers, we found IL-6 in small vesicle structures distributed throughout the fibers under basal (resting) conditions. To determine the kinetics of IL-6 secretion, intact quadriceps muscles were transfected with enhanced green fluorescent protein (EGFP)-tagged IL-6 (IL-6-EGFP), and 5 days later anesthetized mice were imaged before and after muscle contractions in situ. Contractions decreased IL-6-EGFP-containing vesicles and protein by 62% (P

AB - Recent studies suggest that interleukin 6 (IL-6) is released from contracting skeletal muscles; however, the cellular origin, secretion kinetics, and signaling mechanisms regulating IL-6 secretion are unknown. To address these questions, we developed imaging methodology to study IL-6 in fixed mouse muscle fibers and in live animals in vivo. Using confocal imaging to visualize endogenous IL-6 protein in fixed muscle fibers, we found IL-6 in small vesicle structures distributed throughout the fibers under basal (resting) conditions. To determine the kinetics of IL-6 secretion, intact quadriceps muscles were transfected with enhanced green fluorescent protein (EGFP)-tagged IL-6 (IL-6-EGFP), and 5 days later anesthetized mice were imaged before and after muscle contractions in situ. Contractions decreased IL-6-EGFP-containing vesicles and protein by 62% (P

KW - Aminoimidazole Carboxamide

KW - Animals

KW - Green Fluorescent Proteins

KW - Interleukin-6

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Microscopy, Confocal

KW - Muscle Contraction

KW - Muscle Fibers, Skeletal

KW - Ribonucleotides

U2 - 10.2337/db12-1261

DO - 10.2337/db12-1261

M3 - Journal article

C2 - 23761105

SN - 0012-1797

VL - 62

SP - 3081

EP - 3092

JO - Diabetes

JF - Diabetes

IS - 9

ER -

Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo

Abstract

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