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Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases

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Harvard

Chang, CXL, Tan, AT, Or, MY, Toh, KY, Lim, PY, Chia, ASE, Froesig, TM, Nadua, KD, Oh, H-LJ, Leong, HN, Hadrup, SR, Gehring, AJ, Tan, Y-J, Bertoletti, A & Grotenbreg, GM 2013, 'Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases', European Journal of Immunology, bind 43, nr. 4, s. 1109-20. https://doi.org/10.1002/eji.201243088

APA

Chang, C. X. L., Tan, A. T., Or, M. Y., Toh, K. Y., Lim, P. Y., Chia, A. S. E., Froesig, T. M., Nadua, K. D., Oh, H-L. J., Leong, H. N., Hadrup, S. R., Gehring, A. J., Tan, Y-J., Bertoletti, A., & Grotenbreg, G. M. (2013). Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases. European Journal of Immunology, 43(4), 1109-20. https://doi.org/10.1002/eji.201243088

CBE

Chang CXL, Tan AT, Or MY, Toh KY, Lim PY, Chia ASE, Froesig TM, Nadua KD, Oh H-LJ, Leong HN, Hadrup SR, Gehring AJ, Tan Y-J, Bertoletti A, Grotenbreg GM. 2013. Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases. European Journal of Immunology. 43(4):1109-20. https://doi.org/10.1002/eji.201243088

MLA

Vancouver

Author

Chang, Cynthia X L ; Tan, Anthony T ; Or, Ming Yan ; Toh, Kai Yee ; Lim, Pei Yiing ; Chia, Adeline S E ; Froesig, Thomas M ; Nadua, Karen D ; Oh, Hsueh-Ling J ; Leong, Hoe Nam ; Hadrup, Sine R ; Gehring, Adam J ; Tan, Yee-Joo ; Bertoletti, Antonio ; Grotenbreg, Gijsbert M. / Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases. I: European Journal of Immunology. 2013 ; Bind 43, Nr. 4. s. 1109-20.

Bibtex

@article{9b484ae3cc8444a6b37e79cb181d1cff,
title = "Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases",
abstract = "Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, respectively. Unique ligands for all 16 HLA types were constructed to provide the desired soluble HLA product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA-based MHC stability assay. HLA tetramers with redirected specificity could detect antigen-specific CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this population-centric HLA library was demonstrated with the characterization of seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, HBV, and DENV. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state-of-the-art epitope prediction. This flow cytometry-based technology therefore holds considerable promise for monitoring clinically relevant antigen-specific T-cell responses in populations of distinct ethnicity.",
keywords = "Amino Acid Sequence, Asian Continental Ancestry Group, CD8-Positive T-Lymphocytes, Epitopes, T-Lymphocyte, HLA Antigens, Humans, Ligands, Molecular Sequence Data, Peptides, Protein Multimerization, Protein Stability, Virus Diseases",
author = "Chang, {Cynthia X L} and Tan, {Anthony T} and Or, {Ming Yan} and Toh, {Kai Yee} and Lim, {Pei Yiing} and Chia, {Adeline S E} and Froesig, {Thomas M} and Nadua, {Karen D} and Oh, {Hsueh-Ling J} and Leong, {Hoe Nam} and Hadrup, {Sine R} and Gehring, {Adam J} and Yee-Joo Tan and Antonio Bertoletti and Grotenbreg, {Gijsbert M}",
note = "{\textcopyright} 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2013",
month = apr,
doi = "10.1002/eji.201243088",
language = "English",
volume = "43",
pages = "1109--20",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "4",

}

RIS

TY - JOUR

T1 - Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases

AU - Chang, Cynthia X L

AU - Tan, Anthony T

AU - Or, Ming Yan

AU - Toh, Kai Yee

AU - Lim, Pei Yiing

AU - Chia, Adeline S E

AU - Froesig, Thomas M

AU - Nadua, Karen D

AU - Oh, Hsueh-Ling J

AU - Leong, Hoe Nam

AU - Hadrup, Sine R

AU - Gehring, Adam J

AU - Tan, Yee-Joo

AU - Bertoletti, Antonio

AU - Grotenbreg, Gijsbert M

N1 - © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2013/4

Y1 - 2013/4

N2 - Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, respectively. Unique ligands for all 16 HLA types were constructed to provide the desired soluble HLA product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA-based MHC stability assay. HLA tetramers with redirected specificity could detect antigen-specific CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this population-centric HLA library was demonstrated with the characterization of seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, HBV, and DENV. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state-of-the-art epitope prediction. This flow cytometry-based technology therefore holds considerable promise for monitoring clinically relevant antigen-specific T-cell responses in populations of distinct ethnicity.

AB - Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, respectively. Unique ligands for all 16 HLA types were constructed to provide the desired soluble HLA product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA-based MHC stability assay. HLA tetramers with redirected specificity could detect antigen-specific CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this population-centric HLA library was demonstrated with the characterization of seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, HBV, and DENV. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state-of-the-art epitope prediction. This flow cytometry-based technology therefore holds considerable promise for monitoring clinically relevant antigen-specific T-cell responses in populations of distinct ethnicity.

KW - Amino Acid Sequence

KW - Asian Continental Ancestry Group

KW - CD8-Positive T-Lymphocytes

KW - Epitopes, T-Lymphocyte

KW - HLA Antigens

KW - Humans

KW - Ligands

KW - Molecular Sequence Data

KW - Peptides

KW - Protein Multimerization

KW - Protein Stability

KW - Virus Diseases

U2 - 10.1002/eji.201243088

DO - 10.1002/eji.201243088

M3 - Journal article

C2 - 23280567

VL - 43

SP - 1109

EP - 1120

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 4

ER -

ID: 39767920