Concentration and in vivo synthesis of cholecystokinin in subcortical regions of the rat brain

N R Goltermann, J F Rehfeld, H R Petersen

32 Citationer (Scopus)

Abstract

The in vivo biosynthesis of cholecystokinin (CCK) in subcortical regions of the rat brain was studied by intracisternal pulse injections of [35S]methionine. The rats were killed 1.0 or 1.5 h after the injection. Subcortical CCK extracted in boiling water and acetic acid was immunoabsorbed by an antiserum specific for the COOH-terminal sequence of CCK. Gel chromatography of the absorbed CCK separated four molecular forms with elution constants (Kav) of 0.08, 0.50 (corresponding to the tritriacontapeptide amide, CCK-33), 1.10 (corresponding to the COOH-terminal octapeptide, CCK-8), and 1.40 (a component which may correspond to the COOH-terminal tetrapeptide amide). Significant incorporation of [35S]methionine occurred in the largest (Kav approximately 0.08) and octapeptide-like form only. Chasing with methionine indicated a precursor relationship between the largest form and CCK-8. The results demonstrate a substantial synthesis of CCK also in subcortical regions of the brain.

OriginalsprogEngelsk
TidsskriftJournal of Neurochemistry
Vol/bind35
Udgave nummer2
Sider (fra-til)479-83
Antal sider5
ISSN0022-3042
DOI
StatusUdgivet - aug. 1980
Udgivet eksterntJa

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