Abstract
The in vivo biosynthesis of cholecystokinin (CCK) in subcortical regions of the rat brain was studied by intracisternal pulse injections of [35S]methionine. The rats were killed 1.0 or 1.5 h after the injection. Subcortical CCK extracted in boiling water and acetic acid was immunoabsorbed by an antiserum specific for the COOH-terminal sequence of CCK. Gel chromatography of the absorbed CCK separated four molecular forms with elution constants (Kav) of 0.08, 0.50 (corresponding to the tritriacontapeptide amide, CCK-33), 1.10 (corresponding to the COOH-terminal octapeptide, CCK-8), and 1.40 (a component which may correspond to the COOH-terminal tetrapeptide amide). Significant incorporation of [35S]methionine occurred in the largest (Kav approximately 0.08) and octapeptide-like form only. Chasing with methionine indicated a precursor relationship between the largest form and CCK-8. The results demonstrate a substantial synthesis of CCK also in subcortical regions of the brain.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Journal of Neurochemistry |
Vol/bind | 35 |
Udgave nummer | 2 |
Sider (fra-til) | 479-83 |
Antal sider | 5 |
ISSN | 0022-3042 |
DOI | |
Status | Udgivet - aug. 1980 |
Udgivet eksternt | Ja |