Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital

Comparison of styrene-divinylbenzene-based monoliths and Vydac nano-liquid chromatography columns for protein analysis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Gut microbiota profile and selected plasma metabolites in type 1 diabetes without and with stratification by albuminuria

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Circulating Metabolites and Lipids Are Associated to Diabetic Retinopathy in Individuals With Type 1 Diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Integrated lipidomics and proteomics network analysis highlights lipid and immunity pathways associated with Alzheimer's disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Circulating metabolites in relation to presence and development of diabetic retinopathy in individuals with type 1 diabetes

    Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningpeer review

Vis graf over relationer

This report details the fabrication of polystyrene-based nano-LC monolithic columns for the separation of protein molecules. The report focuses on the practical advantages of monolithic columns when compared to conventional packed columns. Capillary columns were made to 100 and 50 microm i.d. and used to analyse a mixture of proteins, these separations were then compared with a conventional protein phase under the same conditions. A second functionalised monolithic polystyrene-based column was also manufactured and compared for the analysis of proteins, under the same analytical conditions, to the standard non-functionalised bare polystyrene monolith. Nano-LC polystyrene monolithic columns were found to be advantageous to conventional phases for the analysis of protein molecules, with a one-step fabrication process, faster analysis times, lower limits of detection hence higher sensitivity.

TidsskriftJournal of Chromatography
Udgave nummer1-2
Sider (fra-til)195-200
Antal sider6
StatusUdgivet - 19 mar. 2004
Eksternt udgivetJa

ID: 54963082