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Region Hovedstaden - en del af Københavns Universitetshospital
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Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

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Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.
OriginalsprogEngelsk
TidsskriftB M C Microbiology
Vol/bind11
Sider (fra-til)73
ISSN1471-2180
DOI
StatusUdgivet - 2011

ID: 33265980