Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

Birgitte Smith; Susan Bodé; Bodil L Petersen; Tim K Jensen; Christian Pipper; Julie Kloppenborg; Mette Boyé; Karen Krogfelt; Lars Mølbak

doi:10.1186/1471-2180-11-73

Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

Birgitte Smith, Susan Bodé, Bodil L Petersen, Tim K Jensen, Christian Pipper, Julie Kloppenborg, Mette Boyé, Karen Krogfelt, Lars Mølbak

79 Citationer (Scopus)

Abstract

Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.

Originalsprog	Engelsk
Tidsskrift	B M C Microbiology
Vol/bind	11
Sider (fra-til)	73
ISSN	1471-2180
DOI	https://doi.org/10.1186/1471-2180-11-73
Status	Udgivet - 2011

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10.1186/1471-2180-11-73

Citationsformater

@article{42d28868380040dc825bc886ab3f2dcb,

title = "Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis",

abstract = "Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.",

keywords = "Bacteria, Biodiversity, DNA, Bacterial, DNA, Ribosomal, Enterocolitis, Necrotizing, Female, Gastrointestinal Tract, Humans, In Situ Hybridization, Fluorescence, Infant, Newborn, Male, RNA, Ribosomal, 16S, Sequence Analysis, DNA",

author = "Birgitte Smith and Susan Bod{\'e} and Petersen, {Bodil L} and Jensen, {Tim K} and Christian Pipper and Julie Kloppenborg and Mette Boy{\'e} and Karen Krogfelt and Lars M{\o}lbak",

year = "2011",

doi = "10.1186/1471-2180-11-73",

language = "English",

volume = "11",

pages = "73",

journal = "B M C Microbiology",

issn = "1471-2180",

publisher = "BioMed Central Ltd",

}

TY - JOUR

T1 - Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

AU - Smith, Birgitte

AU - Bodé, Susan

AU - Petersen, Bodil L

AU - Jensen, Tim K

AU - Pipper, Christian

AU - Kloppenborg, Julie

AU - Boyé, Mette

AU - Krogfelt, Karen

AU - Mølbak, Lars

PY - 2011

Y1 - 2011

N2 - Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.

AB - Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n=24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes.

KW - Bacteria

KW - Biodiversity

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Enterocolitis, Necrotizing

KW - Female

KW - Gastrointestinal Tract

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Infant, Newborn

KW - Male

KW - RNA, Ribosomal, 16S

KW - Sequence Analysis, DNA

U2 - 10.1186/1471-2180-11-73

DO - 10.1186/1471-2180-11-73

M3 - Journal article

C2 - 21486476

SN - 1471-2180

VL - 11

SP - 73

JO - B M C Microbiology

JF - B M C Microbiology

ER -

Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

Abstract

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