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Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity

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Hansen, S, Selman, L, Palaniyar, N, Ziegler, K, Brandt, J, Kliem, A, Jonasson, M, Skjoedt, M-O, Nielsen, O, Hartshorn, K, Jørgensen, TJD, Skjødt, K & Holmskov, U 2010, 'Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity' Journal of immunology (Baltimore, Md. : 1950), bind 185, nr. 10, s. 6096-104. https://doi.org/10.4049/jimmunol.1002185

APA

CBE

Hansen S, Selman L, Palaniyar N, Ziegler K, Brandt J, Kliem A, Jonasson M, Skjoedt M-O, Nielsen O, Hartshorn K, Jørgensen TJD, Skjødt K, Holmskov U. 2010. Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity. Journal of immunology (Baltimore, Md. : 1950). 185(10):6096-104. https://doi.org/10.4049/jimmunol.1002185

MLA

Vancouver

Author

Hansen, Søren ; Selman, Lana ; Palaniyar, Nades ; Ziegler, Karel ; Brandt, Jette ; Kliem, Anette ; Jonasson, Maiken ; Skjoedt, Mikkel-Ole ; Nielsen, Ole ; Hartshorn, Kevan ; Jørgensen, Thomas J D ; Skjødt, Karsten ; Holmskov, Uffe. / Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity. I: Journal of immunology (Baltimore, Md. : 1950). 2010 ; Bind 185, Nr. 10. s. 6096-104.

Bibtex

@article{f3e8800288bb4f3990829cc92e9e40ff,
title = "Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity",
abstract = "Collectins play important roles in the innate immune defense against microorganisms. Recently, a new collectin, collectin 11 (CL-11 or CL-K1), was identified via database searches. In present work, we characterize the structural and functional properties of CL-11. Under nonreducing conditions, in gel permeation chromatography recombinant CL-11 forms disulfide-linked oligomers of 100 and 200 kDa. A mAb-based ELISA estimates the concentration of CL-11 in plasma to be 2.1 μg/ml, and the presence of CL-11 in plasma was further verified by Western blotting and mass spectrometry. Mannan-binding lectin-associated serine protease 1 (MASP-1) copurified with CL-11 and the interaction in plasma with MASP-1 and/or MASP-3 was further demonstrated using ELISA. We identified the adrenal glands, the kidneys, and the liver as primary sites of expression. CL-11 lectin activity was demonstrated by ELISA and showed that CL-11 has preference for l-fucose and d-mannose. We finally show that CL-11 binds to intact bacteria, fungi, and viruses and that CL-11 decreases influenza A virus infectivity and forms complexes with DNA. On the basis of the significant concentration of CL-11 in circulation and CL-11's interaction with various microorganisms and MASP-1 and/or MASP-3, it is conceivable that CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Chromatography, Gel, Collectins, Enzyme-Linked Immunosorbent Assay, Humans, Immunity, Innate, Immunohistochemistry, Mannose-Binding Protein-Associated Serine Proteases, Mass Spectrometry, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Journal Article, Research Support, Non-U.S. Gov't",
author = "S{\o}ren Hansen and Lana Selman and Nades Palaniyar and Karel Ziegler and Jette Brandt and Anette Kliem and Maiken Jonasson and Mikkel-Ole Skjoedt and Ole Nielsen and Kevan Hartshorn and J{\o}rgensen, {Thomas J D} and Karsten Skj{\o}dt and Uffe Holmskov",
year = "2010",
month = "11",
day = "15",
doi = "10.4049/jimmunol.1002185",
language = "English",
volume = "185",
pages = "6096--104",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "10",

}

RIS

TY - JOUR

T1 - Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity

AU - Hansen, Søren

AU - Selman, Lana

AU - Palaniyar, Nades

AU - Ziegler, Karel

AU - Brandt, Jette

AU - Kliem, Anette

AU - Jonasson, Maiken

AU - Skjoedt, Mikkel-Ole

AU - Nielsen, Ole

AU - Hartshorn, Kevan

AU - Jørgensen, Thomas J D

AU - Skjødt, Karsten

AU - Holmskov, Uffe

PY - 2010/11/15

Y1 - 2010/11/15

N2 - Collectins play important roles in the innate immune defense against microorganisms. Recently, a new collectin, collectin 11 (CL-11 or CL-K1), was identified via database searches. In present work, we characterize the structural and functional properties of CL-11. Under nonreducing conditions, in gel permeation chromatography recombinant CL-11 forms disulfide-linked oligomers of 100 and 200 kDa. A mAb-based ELISA estimates the concentration of CL-11 in plasma to be 2.1 μg/ml, and the presence of CL-11 in plasma was further verified by Western blotting and mass spectrometry. Mannan-binding lectin-associated serine protease 1 (MASP-1) copurified with CL-11 and the interaction in plasma with MASP-1 and/or MASP-3 was further demonstrated using ELISA. We identified the adrenal glands, the kidneys, and the liver as primary sites of expression. CL-11 lectin activity was demonstrated by ELISA and showed that CL-11 has preference for l-fucose and d-mannose. We finally show that CL-11 binds to intact bacteria, fungi, and viruses and that CL-11 decreases influenza A virus infectivity and forms complexes with DNA. On the basis of the significant concentration of CL-11 in circulation and CL-11's interaction with various microorganisms and MASP-1 and/or MASP-3, it is conceivable that CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms.

AB - Collectins play important roles in the innate immune defense against microorganisms. Recently, a new collectin, collectin 11 (CL-11 or CL-K1), was identified via database searches. In present work, we characterize the structural and functional properties of CL-11. Under nonreducing conditions, in gel permeation chromatography recombinant CL-11 forms disulfide-linked oligomers of 100 and 200 kDa. A mAb-based ELISA estimates the concentration of CL-11 in plasma to be 2.1 μg/ml, and the presence of CL-11 in plasma was further verified by Western blotting and mass spectrometry. Mannan-binding lectin-associated serine protease 1 (MASP-1) copurified with CL-11 and the interaction in plasma with MASP-1 and/or MASP-3 was further demonstrated using ELISA. We identified the adrenal glands, the kidneys, and the liver as primary sites of expression. CL-11 lectin activity was demonstrated by ELISA and showed that CL-11 has preference for l-fucose and d-mannose. We finally show that CL-11 binds to intact bacteria, fungi, and viruses and that CL-11 decreases influenza A virus infectivity and forms complexes with DNA. On the basis of the significant concentration of CL-11 in circulation and CL-11's interaction with various microorganisms and MASP-1 and/or MASP-3, it is conceivable that CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Blotting, Western

KW - Chromatography, Gel

KW - Collectins

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Immunity, Innate

KW - Immunohistochemistry

KW - Mannose-Binding Protein-Associated Serine Proteases

KW - Mass Spectrometry

KW - Molecular Sequence Data

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.4049/jimmunol.1002185

DO - 10.4049/jimmunol.1002185

M3 - Journal article

VL - 185

SP - 6096

EP - 6104

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 10

ER -

ID: 51598655