Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial

Yusuke Nishimura; Mikkel Jensen; Jacob Bülow; Thomas Tagmose Thomsen; Takuma Arimitsu; Gerrit van Hall; Satoshi Fujita; Lars Holm

doi:10.1007/s00394-021-02782-y

Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial

Yusuke Nishimura, Mikkel Jensen, Jacob Bülow, Thomas Tagmose Thomsen, Takuma Arimitsu, Gerrit van Hall, Satoshi Fujita, Lars Holm^*

^*Corresponding author af dette arbejde

Abstract

PURPOSE: This study investigates if co-ingestion of cluster dextrin (CDX) augments the appearance of intrinsically labeled meat protein hydrolysate-derived amino acid (D5-phenylalanine), Akt/mTORC1 signaling, and myofibrillar protein fractional synthetic rate (FSR).

METHODS: Ten moderately trained healthy males (age: 21.5 ± 2.1 years, body mass: 75.7 ± 7.6 kg, body mass index (BMI): 22.9 ± 2.1 kg/m2) were included for a double-blinded randomized controlled crossover trial. Either 75 g of CDX or glucose (GLC) was given in conjunction with meat protein hydrolysate (0.6 g protein * FFM-1) following a whole-body resistance exercise. A primed-continuous intravenous infusion of L-[15N]-phenylalanine with serial muscle biopsies and venous blood sampling was performed.

RESULTS: A time × group interaction effect was found for serum D5-phenylalanine enrichment (P < 0.01). Serum EAA and BCAA concentrations showed a main effect for group (P < 0.05). Tmax serum BCAA was greater in CDX as compared to GLC (P < 0.05). However, iAUC of all serum parameters did not differ between CDX and GLC (P > 0.05). Tmax serum EAA showed a trend towards a statistical significance favoring CDX over GLC. The phosphorylation of p70S6KThr389, rpS6Ser240/244, ERK1/2Thr202/Tyr204 was greater in CDX compared to GLC (P < 0.05). However, postprandial myofibrillar FSR did not differ between CDX and GLC (P = 0.17).

CONCLUSION: In moderately trained younger males, co-ingestion of CDX with meat protein hydrolysate does not augment the postprandial amino acid availability or myofibrillar FSR as compared to co-ingestion of GLC during the recovery from a whole-body resistance exercise despite an increased intramuscular signaling.

TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03303729 (registered on October 3, 2017).

Originalsprog	Engelsk
Tidsskrift	European Journal of Nutrition
Vol/bind	61
Udgave nummer	5
Sider (fra-til)	2475-2491
Antal sider	17
ISSN	1436-6207
DOI	https://doi.org/10.1007/s00394-021-02782-y
Status	Udgivet - aug. 2022

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Citationsformater

Nishimura, Y., Jensen, M., Bülow, J., Thomsen, T. T., Arimitsu, T., van Hall, G., Fujita, S., & Holm, L. (2022). Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. European Journal of Nutrition, 61(5), 2475-2491. https://doi.org/10.1007/s00394-021-02782-y

Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. / Nishimura, Yusuke; Jensen, Mikkel; Bülow, Jacob et al.
I: European Journal of Nutrition, Bind 61, Nr. 5, 08.2022, s. 2475-2491.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Nishimura, Y, Jensen, M, Bülow, J, Thomsen, TT, Arimitsu, T, van Hall, G, Fujita, S & Holm, L 2022, 'Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial', European Journal of Nutrition, bind 61, nr. 5, s. 2475-2491. https://doi.org/10.1007/s00394-021-02782-y

Nishimura Y, Jensen M, Bülow J, Thomsen TT, Arimitsu T, van Hall G et al. Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. European Journal of Nutrition. 2022 aug.;61(5):2475-2491. doi: 10.1007/s00394-021-02782-y

Nishimura, Yusuke ; Jensen, Mikkel ; Bülow, Jacob et al. / Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males : a double-blinded randomized controlled crossover trial. I: European Journal of Nutrition. 2022 ; Bind 61, Nr. 5. s. 2475-2491.

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title = "Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial",

abstract = "PURPOSE: This study investigates if co-ingestion of cluster dextrin (CDX) augments the appearance of intrinsically labeled meat protein hydrolysate-derived amino acid (D5-phenylalanine), Akt/mTORC1 signaling, and myofibrillar protein fractional synthetic rate (FSR).METHODS: Ten moderately trained healthy males (age: 21.5 ± 2.1 years, body mass: 75.7 ± 7.6 kg, body mass index (BMI): 22.9 ± 2.1 kg/m2) were included for a double-blinded randomized controlled crossover trial. Either 75 g of CDX or glucose (GLC) was given in conjunction with meat protein hydrolysate (0.6 g protein * FFM-1) following a whole-body resistance exercise. A primed-continuous intravenous infusion of L-[15N]-phenylalanine with serial muscle biopsies and venous blood sampling was performed.RESULTS: A time × group interaction effect was found for serum D5-phenylalanine enrichment (P < 0.01). Serum EAA and BCAA concentrations showed a main effect for group (P < 0.05). Tmax serum BCAA was greater in CDX as compared to GLC (P < 0.05). However, iAUC of all serum parameters did not differ between CDX and GLC (P > 0.05). Tmax serum EAA showed a trend towards a statistical significance favoring CDX over GLC. The phosphorylation of p70S6KThr389, rpS6Ser240/244, ERK1/2Thr202/Tyr204 was greater in CDX compared to GLC (P < 0.05). However, postprandial myofibrillar FSR did not differ between CDX and GLC (P = 0.17).CONCLUSION: In moderately trained younger males, co-ingestion of CDX with meat protein hydrolysate does not augment the postprandial amino acid availability or myofibrillar FSR as compared to co-ingestion of GLC during the recovery from a whole-body resistance exercise despite an increased intramuscular signaling.TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03303729 (registered on October 3, 2017).",

keywords = "Adult, Amino Acids, Cross-Over Studies, Dextrins, Eating, Humans, Male, Muscle Proteins, Muscle, Skeletal/metabolism, Phenylalanine, Protein Hydrolysates/metabolism, Resistance Training, Young Adult",

author = "Yusuke Nishimura and Mikkel Jensen and Jacob B{\"u}low and Thomsen, {Thomas Tagmose} and Takuma Arimitsu and {van Hall}, Gerrit and Satoshi Fujita and Lars Holm",

note = "{\textcopyright} 2022. The Author(s).",

year = "2022",

month = aug,

doi = "10.1007/s00394-021-02782-y",

language = "English",

volume = "61",

pages = "2475--2491",

journal = "European Journal of Nutrition",

issn = "1436-6207",

publisher = "Dr. Dietrich/Steinkopff Verlag",

number = "5",

}

TY - JOUR

T1 - Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males

T2 - a double-blinded randomized controlled crossover trial

AU - Nishimura, Yusuke

AU - Jensen, Mikkel

AU - Bülow, Jacob

AU - Thomsen, Thomas Tagmose

AU - Arimitsu, Takuma

AU - van Hall, Gerrit

AU - Fujita, Satoshi

AU - Holm, Lars

PY - 2022/8

Y1 - 2022/8

N2 - PURPOSE: This study investigates if co-ingestion of cluster dextrin (CDX) augments the appearance of intrinsically labeled meat protein hydrolysate-derived amino acid (D5-phenylalanine), Akt/mTORC1 signaling, and myofibrillar protein fractional synthetic rate (FSR).METHODS: Ten moderately trained healthy males (age: 21.5 ± 2.1 years, body mass: 75.7 ± 7.6 kg, body mass index (BMI): 22.9 ± 2.1 kg/m2) were included for a double-blinded randomized controlled crossover trial. Either 75 g of CDX or glucose (GLC) was given in conjunction with meat protein hydrolysate (0.6 g protein * FFM-1) following a whole-body resistance exercise. A primed-continuous intravenous infusion of L-[15N]-phenylalanine with serial muscle biopsies and venous blood sampling was performed.RESULTS: A time × group interaction effect was found for serum D5-phenylalanine enrichment (P < 0.01). Serum EAA and BCAA concentrations showed a main effect for group (P < 0.05). Tmax serum BCAA was greater in CDX as compared to GLC (P < 0.05). However, iAUC of all serum parameters did not differ between CDX and GLC (P > 0.05). Tmax serum EAA showed a trend towards a statistical significance favoring CDX over GLC. The phosphorylation of p70S6KThr389, rpS6Ser240/244, ERK1/2Thr202/Tyr204 was greater in CDX compared to GLC (P < 0.05). However, postprandial myofibrillar FSR did not differ between CDX and GLC (P = 0.17).CONCLUSION: In moderately trained younger males, co-ingestion of CDX with meat protein hydrolysate does not augment the postprandial amino acid availability or myofibrillar FSR as compared to co-ingestion of GLC during the recovery from a whole-body resistance exercise despite an increased intramuscular signaling.TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03303729 (registered on October 3, 2017).

AB - PURPOSE: This study investigates if co-ingestion of cluster dextrin (CDX) augments the appearance of intrinsically labeled meat protein hydrolysate-derived amino acid (D5-phenylalanine), Akt/mTORC1 signaling, and myofibrillar protein fractional synthetic rate (FSR).METHODS: Ten moderately trained healthy males (age: 21.5 ± 2.1 years, body mass: 75.7 ± 7.6 kg, body mass index (BMI): 22.9 ± 2.1 kg/m2) were included for a double-blinded randomized controlled crossover trial. Either 75 g of CDX or glucose (GLC) was given in conjunction with meat protein hydrolysate (0.6 g protein * FFM-1) following a whole-body resistance exercise. A primed-continuous intravenous infusion of L-[15N]-phenylalanine with serial muscle biopsies and venous blood sampling was performed.RESULTS: A time × group interaction effect was found for serum D5-phenylalanine enrichment (P < 0.01). Serum EAA and BCAA concentrations showed a main effect for group (P < 0.05). Tmax serum BCAA was greater in CDX as compared to GLC (P < 0.05). However, iAUC of all serum parameters did not differ between CDX and GLC (P > 0.05). Tmax serum EAA showed a trend towards a statistical significance favoring CDX over GLC. The phosphorylation of p70S6KThr389, rpS6Ser240/244, ERK1/2Thr202/Tyr204 was greater in CDX compared to GLC (P < 0.05). However, postprandial myofibrillar FSR did not differ between CDX and GLC (P = 0.17).CONCLUSION: In moderately trained younger males, co-ingestion of CDX with meat protein hydrolysate does not augment the postprandial amino acid availability or myofibrillar FSR as compared to co-ingestion of GLC during the recovery from a whole-body resistance exercise despite an increased intramuscular signaling.TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03303729 (registered on October 3, 2017).

KW - Adult

KW - Amino Acids

KW - Cross-Over Studies

KW - Dextrins

KW - Eating

KW - Humans

KW - Male

KW - Muscle Proteins

KW - Muscle, Skeletal/metabolism

KW - Phenylalanine

KW - Protein Hydrolysates/metabolism

KW - Resistance Training

KW - Young Adult

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U2 - 10.1007/s00394-021-02782-y

DO - 10.1007/s00394-021-02782-y

M3 - Journal article

C2 - 35182194

SN - 1436-6207

VL - 61

SP - 2475

EP - 2491

JO - European Journal of Nutrition

JF - European Journal of Nutrition

IS - 5

ER -