TY - JOUR
T1 - Cleaning protocols in forensic genetic laboratories
AU - Kampmann, Marie-Louise
AU - Tfelt-Hansen, Jacob
AU - Børsting, Claus
N1 - © 2024. The Author(s).
PY - 2024/9
Y1 - 2024/9
N2 - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD4L did not.
AB - It is pivotal to avoid cross-sample contamination in forensic genetic laboratories and optimal cleaning protocols for the removal of DNA are essential. A survey was performed, and ten forensic genetic laboratories shared their cleaning protocols in pre-PCR and post-PCR laboratories. The cleaning frequencies on different surface areas were somewhat similar, whereas none of the laboratories used the same cleaning reagents. Therefore, the efficiencies of the cleaning protocol utilised were tested and compared. The results showed that freshly made household bleach and Virkon® removed all amplifiable DNA from the surfaces, whereas DNA AWAY™ and the disinfection reagents ethanol, isopropanol, and ChemGene HLD4L did not.
KW - Humans
KW - Laboratories
KW - Polymerase Chain Reaction
KW - Disinfectants
KW - Specimen Handling/methods
KW - 2-Propanol
KW - Forensic Genetics/methods
KW - DNA/isolation & purification
KW - Ethanol
KW - Disinfection/methods
KW - Sodium Hypochlorite
KW - Equipment Contamination/prevention & control
KW - DNA Contamination
UR - http://www.scopus.com/inward/record.url?scp=85191093924&partnerID=8YFLogxK
U2 - 10.1007/s00414-024-03232-0
DO - 10.1007/s00414-024-03232-0
M3 - Journal article
C2 - 38649547
SN - 0937-9827
VL - 138
SP - 1787
EP - 1790
JO - International Journal of Legal Medicine (Print)
JF - International Journal of Legal Medicine (Print)
IS - 5
ER -