TY - JOUR
T1 - Choroid plexus-mediated CSF secretion remains stable in aging rats via high and age-resistant metabolic activity
AU - Lolansen, Sara D
AU - Révész, Eszter O
AU - Andreassen, Søren N
AU - Trapp, Marleen
AU - Salio, Chiara
AU - Sassoé-Pognetto, Marco
AU - Andersen, Jens Velde
AU - Westi, Emil W
AU - Toft-Bertelsen, Trine L
AU - Wardman, Jonathan H
AU - Lundby, Anne-Kristine Meinild
AU - Dela, Flemming
AU - Patrizi, Annarita
AU - Aldana, Blanca I
AU - MacAulay, Nanna
N1 - © 2025. The Author(s).
PY - 2025/7/23
Y1 - 2025/7/23
N2 - Our brain is bathed in cerebrospinal fluid (CSF) that is produced by the choroid plexus. CSF serves as a dispersion route for hormones and nutrients, and a conduit for waste clearance. Age-dependent reduction in the CSF secretion rate could influence cerebral waste clearance and thereby promote cognitive deficits in the elderly. Here, we resolve age-dependent modulation of CSF dynamics and choroid plexus function by complementary in vivo determinations of intracranial pressure (ICP) and CSF secretion/absorption rates, combined with transcriptomic, morphological, and metabolic analysis of choroid plexus in aging male rats. ICP and CSF secretion rate and absorption capacity remain stable with age, and the choroid plexus retains its morphology, structural integrity, gene expression, and high metabolic rate across the tested ages. This work supports the significance of choroid plexus function for brain aging and promotes this tissue as a future target for neurodegenerative diseases associated with impaired waste clearance and cognitive decline.
AB - Our brain is bathed in cerebrospinal fluid (CSF) that is produced by the choroid plexus. CSF serves as a dispersion route for hormones and nutrients, and a conduit for waste clearance. Age-dependent reduction in the CSF secretion rate could influence cerebral waste clearance and thereby promote cognitive deficits in the elderly. Here, we resolve age-dependent modulation of CSF dynamics and choroid plexus function by complementary in vivo determinations of intracranial pressure (ICP) and CSF secretion/absorption rates, combined with transcriptomic, morphological, and metabolic analysis of choroid plexus in aging male rats. ICP and CSF secretion rate and absorption capacity remain stable with age, and the choroid plexus retains its morphology, structural integrity, gene expression, and high metabolic rate across the tested ages. This work supports the significance of choroid plexus function for brain aging and promotes this tissue as a future target for neurodegenerative diseases associated with impaired waste clearance and cognitive decline.
KW - Animals
KW - Choroid Plexus/metabolism
KW - Aging/metabolism
KW - Male
KW - Rats
KW - Cerebrospinal Fluid/metabolism
KW - Intracranial Pressure/physiology
KW - Brain/metabolism
KW - Transcriptome
UR - https://www.scopus.com/pages/publications/105011355025
U2 - 10.1038/s41467-025-61889-6
DO - 10.1038/s41467-025-61889-6
M3 - Journal article
C2 - 40701965
SN - 2041-1722
VL - 16
SP - 6778
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6778
ER -