Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries

Kristian Agmund Haanes; Lars Edvinsson

doi:10.1016/j.febslet.2014.05.044

Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries

Kristian Agmund Haanes, Lars Edvinsson

Klinisk Eksperimentel Forskningsafdeling, Glostrup

14 Citationer (Scopus)

Abstract

Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.

Originalsprog	Engelsk
Tidsskrift	F E B S Letters
Vol/bind	588
Udgave nummer	17
Sider (fra-til)	2936-43
Antal sider	8
ISSN	0014-5793
DOI	https://doi.org/10.1016/j.febslet.2014.05.044
Status	Udgivet - 25 aug. 2014

Adgang til dokumentet

10.1016/j.febslet.2014.05.044

Citationsformater

@article{87c97514a6754a31998d3767afaf377d,

title = "Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries",

abstract = "Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.",

keywords = "Animals, Cerebral Arteries, Coronary Vessels, Female, In Vitro Techniques, Male, Mice, Mice, Inbred BALB C, Muscle Contraction, Pertussis Toxin, Receptors, Purinergic P2Y, Up-Regulation, Uridine Diphosphate Glucose",

author = "Haanes, {Kristian Agmund} and Lars Edvinsson",

year = "2014",

month = aug,

day = "25",

doi = "10.1016/j.febslet.2014.05.044",

language = "English",

volume = "588",

pages = "2936--43",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "17",

}

TY - JOUR

T1 - Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries

AU - Haanes, Kristian Agmund

AU - Edvinsson, Lars

PY - 2014/8/25

Y1 - 2014/8/25

N2 - Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.

AB - Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.

KW - Animals

KW - Cerebral Arteries

KW - Coronary Vessels

KW - Female

KW - In Vitro Techniques

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Muscle Contraction

KW - Pertussis Toxin

KW - Receptors, Purinergic P2Y

KW - Up-Regulation

KW - Uridine Diphosphate Glucose

U2 - 10.1016/j.febslet.2014.05.044

DO - 10.1016/j.febslet.2014.05.044

M3 - Journal article

C2 - 24911208

SN - 0014-5793

VL - 588

SP - 2936

EP - 2943

JO - F E B S Letters

JF - F E B S Letters

IS - 17

ER -

Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater