Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

A M Andersson; H Gaardsvoll; E Giladi; B Dahl; Elisabeth Marianne Bock

Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

A M Andersson, H Gaardsvoll, E Giladi, B Dahl, Elisabeth Marianne Bock

Afdeling for Led- og Knoglekirurgi

18 Citationer (Scopus)

Abstract

A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

Originalsprog	Engelsk
Tidsskrift	F E B S Letters
Vol/bind	263
Udgave nummer	2
Sider (fra-til)	385-8
Antal sider	4
ISSN	0014-5793
Status	Udgivet - 24 apr. 1990

Citationsformater

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title = "Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes",

abstract = "A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.",

keywords = "Animals, Blotting, Northern, Brain Chemistry, Cell Adhesion Molecules, Neuronal, DNA Probes, Exons, Liver, Nucleic Acid Hybridization, Oligonucleotide Probes, RNA, Messenger, Rats",

author = "Andersson, {A M} and H Gaardsvoll and E Giladi and B Dahl and Bock, {Elisabeth Marianne}",

year = "1990",

month = apr,

day = "24",

language = "English",

volume = "263",

pages = "385--8",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

AU - Andersson, A M

AU - Gaardsvoll, H

AU - Giladi, E

AU - Dahl, B

AU - Bock, Elisabeth Marianne

PY - 1990/4/24

Y1 - 1990/4/24

N2 - A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

AB - A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

KW - Animals

KW - Blotting, Northern

KW - Brain Chemistry

KW - Cell Adhesion Molecules, Neuronal

KW - DNA Probes

KW - Exons

KW - Liver

KW - Nucleic Acid Hybridization

KW - Oligonucleotide Probes

KW - RNA, Messenger

KW - Rats

M3 - Journal article

C2 - 2335244

SN - 0014-5793

VL - 263

SP - 385

EP - 388

JO - F E B S Letters

JF - F E B S Letters

IS - 2

ER -

Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

Abstract

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