Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein

Katrine T Callesen*, Sofia Mogren, Frida Berlin, Cecilia Andersson, Susanne Schmidt, Lotte Klitfod, Vanesa Esteban, Lars K Poulsen, Bettina M Jensen

*Corresponding author af dette arbejde

Abstrakt

Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64% MCT). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcεRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.

OriginalsprogEngelsk
Artikelnummer1062
TidsskriftBiomedicines
Vol/bind10
Udgave nummer5
ISSN2227-9059
DOI
StatusUdgivet - 3 maj 2022

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