Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

Jeppe Madura Larsen; Carsten Geisler; Martin Weiss Nielsen; Lasse Boding; Marina Von Essen; Ann Kathrine Hansen; Lone Skov; Charlotte Menné Bonefeld

doi:10.1111/j.1600-0536.2007.01230.x

Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

Jeppe Madura Larsen, Carsten Geisler, Martin Weiss Nielsen, Lasse Boding, Marina Von Essen, Ann Kathrine Hansen, Lone Skov, Charlotte Menné Bonefeld

Allergi, Hud- og Kønssygdomme

24 Citationer (Scopus)

Abstrakt

BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.

OBJECTIVES: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.

PATIENTS/METHODS: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.

RESULTS: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.

CONCLUSIONS: Our study support recent findings that several cell types seem to be involved in CHS.

Originalsprog	Engelsk
Tidsskrift	Contact Dermatitis
Vol/bind	57
Udgave nummer	5
Sider (fra-til)	300-8
Antal sider	9
ISSN	0105-1873
DOI	https://doi.org/10.1111/j.1600-0536.2007.01230.x
Status	Udgivet - nov. 2007

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10.1111/j.1600-0536.2007.01230.x

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@article{1281524b0e0f480289d5160c40b5e707,

title = "Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity",

abstract = "BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.OBJECTIVES: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.PATIENTS/METHODS: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.RESULTS: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.CONCLUSIONS: Our study support recent findings that several cell types seem to be involved in CHS.",

keywords = "Allergens, Animals, Antibodies, Dermatitis, Allergic Contact, Dinitrofluorobenzene, Female, Flow Cytometry, Lymph Nodes, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Oxazolone, T-Lymphocytes",

author = "Larsen, {Jeppe Madura} and Carsten Geisler and Nielsen, {Martin Weiss} and Lasse Boding and {Von Essen}, Marina and Hansen, {Ann Kathrine} and Lone Skov and Bonefeld, {Charlotte Menn{\'e}}",

year = "2007",

month = nov,

doi = "10.1111/j.1600-0536.2007.01230.x",

language = "English",

volume = "57",

pages = "300--8",

journal = "Contact Dermatitis",

issn = "0105-1873",

publisher = "Wiley-Blackwell Munksgaard",

number = "5",

}

TY - JOUR

T1 - Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

AU - Larsen, Jeppe Madura

AU - Geisler, Carsten

AU - Nielsen, Martin Weiss

AU - Boding, Lasse

AU - Von Essen, Marina

AU - Hansen, Ann Kathrine

AU - Skov, Lone

AU - Bonefeld, Charlotte Menné

PY - 2007/11

Y1 - 2007/11

N2 - BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.OBJECTIVES: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.PATIENTS/METHODS: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.RESULTS: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.CONCLUSIONS: Our study support recent findings that several cell types seem to be involved in CHS.

AB - BACKGROUND: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.OBJECTIVES: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.PATIENTS/METHODS: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.RESULTS: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.CONCLUSIONS: Our study support recent findings that several cell types seem to be involved in CHS.

KW - Allergens

KW - Animals

KW - Antibodies

KW - Dermatitis, Allergic Contact

KW - Dinitrofluorobenzene

KW - Female

KW - Flow Cytometry

KW - Lymph Nodes

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred C57BL

KW - Oxazolone

KW - T-Lymphocytes

U2 - 10.1111/j.1600-0536.2007.01230.x

DO - 10.1111/j.1600-0536.2007.01230.x

M3 - Journal article

C2 - 17937744

VL - 57

SP - 300

EP - 308

JO - Contact Dermatitis

JF - Contact Dermatitis

SN - 0105-1873

IS - 5

ER -

Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

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