Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

Díaz-García, J, Arendrup, MC, Cantón, R, García-Rodríguez, J, Gómez, A, Gómez, E, Orden, B, Parisi, G, Pemán, J, Posteraro, B, Sanguinetti, M, Da Matta, DA, Colombo, AL, Muñoz, P, Sánchez-Carrillo, C, Guinea, J & Escribano, P 2020, 'Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†', Medical Mycology, bind 58, nr. 7, s. 887-895. https://doi.org/10.1093/mmy/myaa002

APA

Díaz-García, J., Arendrup, M. C., Cantón, R., García-Rodríguez, J., Gómez, A., Gómez, E., Orden, B., Parisi, G., Pemán, J., Posteraro, B., Sanguinetti, M., Da Matta, D. A., Colombo, A. L., Muñoz, P., Sánchez-Carrillo, C., Guinea, J., & Escribano, P. (2020). Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†. Medical Mycology, 58(7), 887-895. https://doi.org/10.1093/mmy/myaa002

CBE

Díaz-García J, Arendrup MC, Cantón R, García-Rodríguez J, Gómez A, Gómez E, Orden B, Parisi G, Pemán J, Posteraro B, Sanguinetti M, Da Matta DA, Colombo AL, Muñoz P, Sánchez-Carrillo C, Guinea J, Escribano P. 2020. Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†. Medical Mycology. 58(7):887-895. https://doi.org/10.1093/mmy/myaa002

MLA

Vancouver

Author

Díaz-García, Judith ; Arendrup, Maiken C ; Cantón, Rafael ; García-Rodríguez, Julio ; Gómez, Ana ; Gómez, Elia ; Orden, Beatriz ; Parisi, Gabriella ; Pemán, Javier ; Posteraro, Brunella ; Sanguinetti, Maurizio ; Da Matta, Daniel Archimedes ; Colombo, Arnaldo L ; Muñoz, Patricia ; Sánchez-Carrillo, Carlos ; Guinea, Jesús ; Escribano, Pilar. / Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†. I: Medical Mycology. 2020 ; Bind 58, Nr. 7. s. 887-895.

Bibtex

@article{87702651378d4575b2b9bc22f9c62fab,
title = "Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†",
abstract = "The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries-and the same number of singleton genotypes used as controls-were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters-particularly in the case of C. albicans-show significantly more biomass production and metabolic activity than singleton genotypes.",
author = "Judith D{\'i}az-Garc{\'i}a and Arendrup, {Maiken C} and Rafael Cant{\'o}n and Julio Garc{\'i}a-Rodr{\'i}guez and Ana G{\'o}mez and Elia G{\'o}mez and Beatriz Orden and Gabriella Parisi and Javier Pem{\'a}n and Brunella Posteraro and Maurizio Sanguinetti and {Da Matta}, {Daniel Archimedes} and Colombo, {Arnaldo L} and Patricia Mu{\~n}oz and Carlos S{\'a}nchez-Carrillo and Jes{\'u}s Guinea and Pilar Escribano",
note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.",
year = "2020",
month = oct,
day = "1",
doi = "10.1093/mmy/myaa002",
language = "English",
volume = "58",
pages = "887--895",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "7",

}

RIS

TY - JOUR

T1 - Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†

AU - Díaz-García, Judith

AU - Arendrup, Maiken C

AU - Cantón, Rafael

AU - García-Rodríguez, Julio

AU - Gómez, Ana

AU - Gómez, Elia

AU - Orden, Beatriz

AU - Parisi, Gabriella

AU - Pemán, Javier

AU - Posteraro, Brunella

AU - Sanguinetti, Maurizio

AU - Da Matta, Daniel Archimedes

AU - Colombo, Arnaldo L

AU - Muñoz, Patricia

AU - Sánchez-Carrillo, Carlos

AU - Guinea, Jesús

AU - Escribano, Pilar

N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries-and the same number of singleton genotypes used as controls-were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters-particularly in the case of C. albicans-show significantly more biomass production and metabolic activity than singleton genotypes.

AB - The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries-and the same number of singleton genotypes used as controls-were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters-particularly in the case of C. albicans-show significantly more biomass production and metabolic activity than singleton genotypes.

U2 - 10.1093/mmy/myaa002

DO - 10.1093/mmy/myaa002

M3 - Journal article

C2 - 32022851

VL - 58

SP - 887

EP - 895

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 7

ER -

ID: 62341385