Ca(2+)-dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils

N Borregaard, L Kjeldsen, K Lollike, H Sengeløv

Abstract

In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca(2+)-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.

OriginalsprogEngelsk
TidsskriftFEBS Letters
Vol/bind304
Udgave nummer2-3
Sider (fra-til)195-7
Antal sider3
ISSN0014-5793
DOI
StatusUdgivet - 15 jun. 1992
Udgivet eksterntJa

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