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Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model

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@article{871c73a7a9cf4ea9beaac96f58236040,
title = "Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model",
abstract = "PURPOSE: It has been proposed that changes in the permeability of Bruch's membrane play a role in the pathogenesis of age-related macular degeneration (AMD). This paper investigates, in an in vivo porcine model, the migration of fluorescent latex beads across the Bruch's membrane after subretinal injection.METHODS: Forty-one healthy eyes of 33 three-month-old domestic pigs received a subretinal injection of 0.5, 1.0, 2.0, or 4.0 μm fluorescent latex beads. Between three hours and five weeks after injection evaluations were performed with fundus photographs and histology. Fluorescent beads were identified in unstained histologic sections using the rhodamine filter with the light microscope.RESULTS: The fluorescent latex beads relocated from the subretinal space. Intact beads up to 2.0 μm were found in the choroid, sclera, and extrascleral space. The smaller beads were also found inside choroidal and extrascleral blood vessels. In contrast, the larger beads of 4.0 μm did not pass the Bruch's membrane.CONCLUSION: Subretinally implanted beads up to 2.0 μm pass the Bruch's membrane intact and cross the blood-ocular barrier. The intact beads are found in the choroid, sclera and inside blood vessels. The results give reason to consider the role of subretinal clearance and passage of Bruch's membrane in the development of AMD.",
keywords = "Aging, Drusen, RPE transplantation, Stem-cell transplantation, Subretinal space, Subretinal surgery, Subretinal transplantation, Rhodamines/metabolism, Sclera/metabolism, Permeability, Intracellular Space, Latex, Particle Size, Microspheres, Animals, Biological Transport, Choroid/metabolism, Female, Models, Animal, Fluorescent Dyes/metabolism, Sus scrofa, Injections, Intraocular, Bruch Membrane/metabolism",
author = "S{\o}rensen, {Nina Buus} and Christiansen, {Anders Tolstrup} and Kj{\ae}r, {Troels Wesenberg} and Kristian Klemp and {la Cour}, Morten and Steffen Heegaard and Kiilgaard, {Jens Folke}",
note = "Copyright {\textcopyright} 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.",
year = "2019",
month = mar,
doi = "10.1016/j.exer.2018.11.019",
language = "English",
volume = "180",
pages = "1--7",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model

AU - Sørensen, Nina Buus

AU - Christiansen, Anders Tolstrup

AU - Kjær, Troels Wesenberg

AU - Klemp, Kristian

AU - la Cour, Morten

AU - Heegaard, Steffen

AU - Kiilgaard, Jens Folke

N1 - Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

PY - 2019/3

Y1 - 2019/3

N2 - PURPOSE: It has been proposed that changes in the permeability of Bruch's membrane play a role in the pathogenesis of age-related macular degeneration (AMD). This paper investigates, in an in vivo porcine model, the migration of fluorescent latex beads across the Bruch's membrane after subretinal injection.METHODS: Forty-one healthy eyes of 33 three-month-old domestic pigs received a subretinal injection of 0.5, 1.0, 2.0, or 4.0 μm fluorescent latex beads. Between three hours and five weeks after injection evaluations were performed with fundus photographs and histology. Fluorescent beads were identified in unstained histologic sections using the rhodamine filter with the light microscope.RESULTS: The fluorescent latex beads relocated from the subretinal space. Intact beads up to 2.0 μm were found in the choroid, sclera, and extrascleral space. The smaller beads were also found inside choroidal and extrascleral blood vessels. In contrast, the larger beads of 4.0 μm did not pass the Bruch's membrane.CONCLUSION: Subretinally implanted beads up to 2.0 μm pass the Bruch's membrane intact and cross the blood-ocular barrier. The intact beads are found in the choroid, sclera and inside blood vessels. The results give reason to consider the role of subretinal clearance and passage of Bruch's membrane in the development of AMD.

AB - PURPOSE: It has been proposed that changes in the permeability of Bruch's membrane play a role in the pathogenesis of age-related macular degeneration (AMD). This paper investigates, in an in vivo porcine model, the migration of fluorescent latex beads across the Bruch's membrane after subretinal injection.METHODS: Forty-one healthy eyes of 33 three-month-old domestic pigs received a subretinal injection of 0.5, 1.0, 2.0, or 4.0 μm fluorescent latex beads. Between three hours and five weeks after injection evaluations were performed with fundus photographs and histology. Fluorescent beads were identified in unstained histologic sections using the rhodamine filter with the light microscope.RESULTS: The fluorescent latex beads relocated from the subretinal space. Intact beads up to 2.0 μm were found in the choroid, sclera, and extrascleral space. The smaller beads were also found inside choroidal and extrascleral blood vessels. In contrast, the larger beads of 4.0 μm did not pass the Bruch's membrane.CONCLUSION: Subretinally implanted beads up to 2.0 μm pass the Bruch's membrane intact and cross the blood-ocular barrier. The intact beads are found in the choroid, sclera and inside blood vessels. The results give reason to consider the role of subretinal clearance and passage of Bruch's membrane in the development of AMD.

KW - Aging

KW - Drusen

KW - RPE transplantation

KW - Stem-cell transplantation

KW - Subretinal space

KW - Subretinal surgery

KW - Subretinal transplantation

KW - Rhodamines/metabolism

KW - Sclera/metabolism

KW - Permeability

KW - Intracellular Space

KW - Latex

KW - Particle Size

KW - Microspheres

KW - Animals

KW - Biological Transport

KW - Choroid/metabolism

KW - Female

KW - Models, Animal

KW - Fluorescent Dyes/metabolism

KW - Sus scrofa

KW - Injections, Intraocular

KW - Bruch Membrane/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85057317286&partnerID=8YFLogxK

U2 - 10.1016/j.exer.2018.11.019

DO - 10.1016/j.exer.2018.11.019

M3 - Journal article

C2 - 30468719

VL - 180

SP - 1

EP - 7

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

ER -

ID: 56470049