Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes From Mothers With Type 1 Diabetes Contain Aberrant Levels of microRNAs

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Distinct Autoimmune Anti-α-Synuclein Antibody Patterns in Multiple System Atrophy and Parkinson’s Disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Peptide Super-Agonist Enhances T-Cell Responses to Melanoma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Complement Nomenclature-Deconvoluted

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Linking glycemic dysregulation in diabetes to symptoms, comorbidities, and genetics through EHR data mining

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Effect of motivational interviewing on gestational weight gain and fetal growth in pregnant women with type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Increased leptin, decreased adiponectin and FGF21 concentrations in adolescent offspring of women with gestational diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. White coat hypertension in early pregnancy in women with pre-existing diabetes: prevalence and pregnancy outcomes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Migration, body mass index and gestational diabetes mellitus among women in Denmark 2004-2015: a register-based study

    Publikation: KonferencebidragKonferenceabstrakt til konferenceForskningpeer review

Vis graf over relationer

The breast milk plays a crucial role in shaping the initial intestinal microbiota and mucosal immunity of the infant. Interestingly, breastfeeding has proven to be protective against the early onset of immune-mediated diseases including type 1 diabetes. Studies have shown that exosomes from human breast milk are enriched in immune-modulating miRNAs suggesting that exosomal miRNAs (exomiRs) transferred to the infant could play a critical role in the development of the infant's immune system. We extracted exomiRs from breast milk of 52 lactating mothers (26 mothers with type 1 diabetes and 26 healthy mothers), to identify any differences in the exomiR content between the two groups. Small RNA-sequencing was performed to identify known and novel miRNAs in both groups. A total of 631 exomiRs were detected by small RNA sequencing including immune-related miRNAs such as hsa-let-7c, hsa-miR-21, hsa-miR-34a, hsa-miR-146b, and hsa-miR-200b. In addition, ~200 novel miRNAs were identified in both type 1 diabetes and control samples. Among the known miRNAs, nine exomiR's were found differentially expressed in mothers with type 1 diabetes compared to healthy mothers. The highly up-regulated miRNAs, hsa-miR-4497, and hsa-miR-3178, increased lipopolysaccharide-induced expression and secretion of tumor necrosis factor α (TNFα) in human monocytes. The up-regulated miRNA target genes were significantly enriched for longevity-regulating pathways and FoxO signaling. Our findings suggest a role of breast milk-derived exomiRs in modulating the infant's immune system.

OriginalsprogEngelsk
Artikelnummer2543
TidsskriftFrontiers in Immunology
Vol/bind10
Sider (fra-til)1-10
Antal sider10
ISSN1664-3224
DOI
StatusUdgivet - okt. 2019

Bibliografisk note

Copyright © 2019 Mirza, Kaur, Nielsen, Størling, Yarani, Roursgaard, Mathiesen, Damm, Svare, Mortensen and Pociot.

ID: 58351358