Binding of a iodinated substance P analog to a NK-1 receptor on isolated cell membranes from rat anterior pituitary

P J Larsen, J D Mikkelsen, T Saermark

42 Citationer (Scopus)

Abstract

The characteristics of binding sites for substance-P (SP) on rat anterior pituitary membranes were studied, using 125I-labeled Bolton-Hunter SP as a ligand. The binding of [125I]Bolton-Hunter SP was saturable and reversible, and reached a plateau of maximal binding after approximately 12 min of incubation. Scatchard and Hill analyses of specific binding revealed a single class of noninteracting binding sites with a high affinity (Kd = 0.72 nM) and a moderate density (binding capacity = 32.16 fmol/mg protein). The biologically active tachykinins, which, in addition to SP, consist of physalaemin, eledoisin, kassinin, neurokinin-A, and neurokinin-B, could inhibit the binding in a concentration-dependent manner. Based on titration curves, the IC50 values of the tachykinins were measured, and the receptor was classified as a NK-1 receptor. These results demonstrate that a population of specific SP-binding sites is present in rat anterior pituitary and further support evidence that this peptide has an influence on pituitary function.

OriginalsprogEngelsk
TidsskriftEndocrinology
Vol/bind124
Udgave nummer5
Sider (fra-til)2548-57
Antal sider10
ISSN0013-7227
DOI
StatusUdgivet - maj 1989
Udgivet eksterntJa

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